Abcam’s anti-Adenovirus IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Adenovirus in Human serum and plasma.
A 96-well plate has been precoated with Adenovirus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Adenovirus-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Adenovirus IgG sample captured in plate.
|Components||Identifier||1 x 96 tests|
|20X Washing Solution||White cap||1 x 50ml|
|Adenovirus (IgG) Coated Microplate (12 x 8 wells)||12 strips of 8 wells||1 unit|
|Adenovirus anti-IgG HRP Conjugate||Colored blue; Black cap||1 x 20ml|
|Adenovirus IgG Cut-off Control||Colored yellow; Green cap||1 x 3ml|
|Adenovirus IgG Negative Control||Colored yellow; Blue cap||1 x 2ml|
|Adenovirus IgG Positive Control||Colored yellow; Red cap||1 x 2ml|
|Cover foil||1 unit|
|IgG Sample Diluent||Colored yellow; White cap||1 x 100ml|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|TMB Substrate Solution||Yellow cap||1 x 15ml|
Our Abpromise guarantee covers the use of ab108705 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent dilution.|
ab108705 has not yet been referenced specifically in any publications.