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Abcam’s anti-Adenovirus IgM Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Adenovirus in Human serum and plasma.
A 96-well plate has been precoated with Adenovirus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Adenovirus-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Adenovirus IgM sample captured in plate.
|Components||Identifier||1 x 96 tests|
|1 Strip holder||1 unit|
|20X Washing Solution||White cap||1 x 50ml|
|Adenovirus (IgM) Coated Microplate (12 x 8 wells)||1 unit|
|Adenovirus anti-IgM HRP Conjugate||Black cap||1 x 20ml|
|Adenovirus IgM Cut-off Control||Green cap||1 x 3ml|
|Adenovirus IgM Negative Control||Blue cap||1 x 2ml|
|Adenovirus IgM Positive Control||Red cap||1 x 2ml|
|Cover foil||1 unit|
|IgM Sample Diluent||White cap||1 x 100ml|
|Stop Solution||1 x 15ml|
|TMB Substrate Solution||Yellow cap||1 x 15ml|
Our Abpromise guarantee covers the use of ab108706 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent concentration.|