Overview

  • Product name
    Human Adiponectin ELISA Kit
    See all Adiponectin kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 25 pg/ml
  • Recovery

    < 96 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 96.59 83% - 107%
    Serum 93.57 83% - 104%
    Plasma 90.29 80% - 103%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Human Adiponectin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Adiponectin in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human Adiponectin coated on a 96-well plate. Standards and samples are pipetted into the wells and Adiponectin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Adiponectin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Adiponectin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimisation may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    80x HRP-Streptavidin Concentrate 1 x 200µl
    Adiponectin Microplate with anti-human Adiponectin. 1 unit
    Assay Diluent A 2 x 30ml
    Assay Diluent C 2 x 15ml
    biotinylated antihuman Adiponectin 2 vials
    Standards recombinant human Adiponectin. 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • Research areas
  • Function
    Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
  • Tissue specificity
    Synthesized exclusively by adipocytes and secreted into plasma.
  • Involvement in disease
    Defects in ADIPOQ are the cause of adiponectin deficiency (ADPND) [MIM:612556]. ADPND results in very low concentrations of plasma adiponectin.
    Genetic variations in ADIPOQ are associated with non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.
  • Sequence similarities
    Contains 1 C1q domain.
    Contains 1 collagen-like domain.
  • Domain
    The C1q domain is commonly called the globular domain.
  • Post-translational
    modifications
    Hydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting.
    HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes.
    O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • 30 kDa adipocyte complement related protein
    • 30 kDa adipocyte complement-related protein
    • ACDC
    • ACRP30
    • ADIPO_HUMAN
    • Adipocyte
    • Adipocyte C1q and collagen domain containing protein
    • Adipocyte complement related 30 kDa protein
    • Adipocyte complement related protein of 30 kDa
    • Adipocyte complement-related 30 kDa protein
    • adipocyte-specific secretory protein
    • Adiponectin
    • Adiponectin precursor
    • adiponectin, C1Q and collagen domain containing
    • Adipoq
    • Adipose most abundant gene transcript 1
    • Adipose most abundant gene transcript 1 protein
    • Adipose specific collagen like factor
    • ADIPQTL1
    • ADPN
    • APM 1
    • apM-1
    • APM1
    • C1q and collagen domain-containing protein
    • GBP28
    • Gelatin binding protein
    • Gelatin binding protein 28
    • Gelatin-binding protein
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab99968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Adiponectin measured in biological fluids showing quantity (pg) per mL of tested sample. Human serum and plasma were diluted 2700-24300 fold. Rat and mouse serum were diluted 1-3 fold. Human urine and saliva were diluted 300-2700 fold.

  • Representative Standard Curve using ab99968

  • Representative Standard Curve using ab99968

Protocols

References

This product has been referenced in:
  • Zhao L  et al. Chitosan oligosaccharide improves the therapeutic efficacy of sitagliptin for the therapy of Chinese elderly patients with type 2 diabetes mellitus. Ther Clin Risk Manag 13:739-750 (2017). Read more (PubMed: 28721055) »
  • Li YC  et al. Relationships of Dietary Histidine and Obesity in Northern Chinese Adults, an Internet-Based Cross-Sectional Study. Nutrients 8:N/A (2016). Human . Read more (PubMed: 27409634) »

See all 5 Publications for this product

Customer reviews and Q&As

Human Adiponectin ELISA Kit testing for rhesus macaques

Excellent Excellent 5/5 (Ease of Use)
Abreviews
This kit is suitable for rhesus macaques. Plasma of 17 rhesus macaques were tested and yielded concentrations comparable to human levels and other published studies of rhesus macaques.
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Abcam user community

Verified customer

Submitted Nov 02 2015

Thank you for contacting us. I sent your inquiry to the lab, they did not send original data but sent the reply below. Please let me know if you have any more questions.



. A 30,000-fold dilution is quite high but our Human IgG ELISA ...

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Thank you for contacting Abcam.

Unfortunately we have not tested pig samples with theab99968 Human Adiponectin ELISA kit, so we do not know the cross reactivity.Both antibodies used in this kit are mouse monoclonal and the epitope recognized ...

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Thank you for your enquiry.

It is possible that high viscosity of the sample can cause interference with the results. However, I would like to state that we have not tested media with such a high fat content and viscosity, therefore we do no...

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I'm happy to help! Please let me know if you need anything further.

The biotinylated anti-human adiponectin antibody is monoclonal and is raised from mice.

I hope this is helpful. Please contact us again if you have any further questions.

Thank you for your reply and for your patience. It has taken a day or two to confirm this information for you.

ab99968 Adiponectin Human ELISA Kitas been successfully tested with EDTA-plasma, heparin-plasma, and citrate-plasma.

Ot...

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Thank you for your recent telephone enquiry.

I can confirm that the matched pair of antibodies for the Human Adiponectin ELISA kit (ab99968) have an epitope in the globular region (aa 104-244). Therefore, this kit will recognize trimers and ...

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Thank you for contacting us. The capture antibody for the Adiponectin Human ELISA Kits are a monoclonal mouse antibody for kit ab99968 and a polyclonal antibody for kit ab108786 I hope this information is helpful to you. Please do not h...

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