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|Sample type||Average %||Range|
|Serum||122.5||105% - 131%|
|Plasma||91.51||78% - 103%|
|Cell culture media||76.17||66% - 87%|
Abcam’s aFGF (acid FGF) Human ELISA Kit (ab193691) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human aFGF in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for human aFGF coated on a 96-well plate. Standards and samples are pipetted into the wells and aFGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human aFGF antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of aFGF bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|5X Assay Diluent||1 x 15ml|
|500X HRP-Streptavidin Concentrate||1 x 200µl|
|Biotinylated Human aFGF detection antibody (lyophilized)||2 vials|
|Human aFGF standards||2 vials|
|Pre-coated Human aFGF Microplate (12 strips x 8 wells)||1 unit|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab193691 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab193691 has not yet been referenced specifically in any publications.