Overview

  • Product name
    Human aFGF ELISA Kit (acid FGF)
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    12 pg/ml
  • Range
    12 pg/ml - 4000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 122.5 105% - 131%
    Plasma 91.51 78% - 103%
    Cell culture media 76.17 66% - 87%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s aFGF (acid FGF) Human ELISA Kit (ab193691) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human aFGF in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for human aFGF coated on a 96-well plate. Standards and samples are pipetted into the wells and aFGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human aFGF antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of aFGF bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent 1 x 15ml
    500X HRP-Streptavidin Concentrate 1 x 200µl
    Biotinylated Human aFGF detection antibody (lyophilized) 2 vials
    Human aFGF standards 2 vials
    Pre-coated Human aFGF Microplate (12 strips x 8 wells) 1 unit
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas
  • Function
    Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro.
  • Tissue specificity
    Predominantly expressed in kidney and brain. Detected at much lower levels in heart and skeletal muscle.
  • Sequence similarities
    Belongs to the heparin-binding growth factors family.
  • Post-translational
    modifications
    In the nucleus, phosphorylated by PKC/PRKCD.
  • Cellular localization
    Secreted. Cytoplasm. Cytoplasm > cell cortex. Cytoplasm > cytosol. Nucleus. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1 (By similarity). Binding of exogenous FGF1 to FGFR facilitates endocytosis followed by translocation of FGF1 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as LRRC59.
  • Information by UniProt
  • Alternative names
    • Acidic fibroblast growth factor
    • aFGF
    • Beta endothelial cell growth factor
    • ECGF
    • Endothelial cell growth factor
    • FGF-1
    • Fgf1
    • FGF1_HUMAN
    • FGFA
    • Fibroblast growth factor 1
    • GLIO703
    • HBGF-1
    • HBGF1
    • Heparin-binding growth factor 1
    see all
  • Database links

    Applications

    Our Abpromise guarantee covers the use of ab193691 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Sandwich ELISA Use at an assay dependent concentration.

    Images

    • Typical Standard Curve
      Typical Standard Curve

      Standard Curve using ab193691 aFGF (acid FGF) Human ELISA Kit.

    Protocols

    References

    ab193691 has not yet been referenced specifically in any publications.

    Publishing research using ab193691? Please let us know so that we can cite the reference in this datasheet.

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