Overview

  • Product name
    Human AIF Profiling ELISA Kit
    See all AIF kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Cell lysates 4 3.6%
    Inter-assay
    Sample n Mean SD CV%
    Cell lysates 3 11.4%
  • Sample type
    Tissue Extracts, Cell Lysate
  • Assay type
    Sandwich (qualitative)
  • Sensitivity
    > 6 µg/ml
  • Range
    6 µg/ml - 250 µg/ml
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Product overview

    Apoptosis-inducing factor is a mitochondrial enzyme with probable oxidoreductase activity which functions to regulate Complex I activity in the mitochondrial membrane. When mitochondria are damaged, as in apoptosis, AIF can migrate to the cytosol and nucleus. AIF in the nucleus signals chromatin condensation and DNA fragmentation. This method of programmed cell death (apoptosis) does not require caspase activation.


     In addition to its role in apoptosis defects in AIF1 are the cause of combined oxidative phosphorylation deficiency type 6 (COXPD6). COXPD6 is a mitochondrial disease resulting in a neurodegenerative disorder characterized by psychomotor delay, hypotonia, areflexia, muscle weakness and wasting.


    Get results in 90 minutes with Human AIF ELISA Kit (Apoptosis-Inducing Factor 1) (ab184858) from our SimpleStep ELISA® range.

  • Tested applications
    Suitable for: ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X AIF Detector Antibody 1 x 1ml
    10X Blocking Buffer 1 x 6ml
    10X HRP Label 1 x 1ml
    20X Buffer 1 x 20ml
    AIF Microplate (12 x 8 antibody coated well strips) 1 unit
    Extraction Buffer 1 x 15ml
    HRP Development Solution 1 x 12ml
  • Research areas
  • Function
    Probable oxidoreductase that has a dual role in controlling cellular life and death; during apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e., caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.
  • Involvement in disease
    Defects in AIFM1 are the cause of combined oxidative phosphorylation deficiency type 6 (COXPD6) [MIM:300816]. It is a mitochondrial disease resulting in a neurodegenerative disorder characterized by psychomotor delay, hypotonia, areflexia, muscle weakness and wasting.
  • Sequence similarities
    Belongs to the FAD-dependent oxidoreductase family.
  • Post-translational
    modifications
    Under normal conditions, a 54-residue N-terminal segment is first proteolytically removed during or just after translocation into the mitochondrial intermembrane space (IMS) by the mitochondrial processing peptidase (MPP) to form the inner-membrane-anchored mature form (AIFmit). During apoptosis, it is further proteolytically processed at amino-acid position 101 leading to the generation of the mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis in a caspase-independent manner.
  • Cellular localization
    Mitochondrion intermembrane space. Mitochondrion inner membrane. Cytoplasm. Nucleus. Cytoplasm > perinuclear region. Proteolytic cleavage during or just after translocation into the mitochondrial intermembrane space (IMS) results in the formation of an inner-membrane-anchored mature form (AIFmit). During apoptosis, further proteolytic processing leads to a mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis. Colocalizes with EIF3G in the nucleus and perinuclear region.
  • Information by UniProt
  • Alternative names
    • AIFM1
    • AIFM1_HUMAN
    • AIFsh2
    • Apoptosis-inducing factor
    • Apoptosis-inducing factor 1
    • Apoptosis-inducing factor 1, mitochondrial
    • Apoptosis-inducing factor, mitochondrion-associated, 1
    • COXPD6
    • mitochondrial
    • PDCD8
    • Programmed cell death 8
    • Programmed cell death protein 8
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab126583 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Images

  • Example serially titrated control sample of HeLa cell extract in the working range of the assay.
  • Anti-AIF antibody [7F7AB10] (ab110327) at 5 µg/ml + Human heart tissue lysate - mitochondrial extract (ab110337) at 5 µg
  • Immunocytochemistry analysis of normal untreated HeLa cells with the capture antibody in this kit, ab110327, at 5µg/ml shows staining of AIF is located exclusively to mitochondria. Nuclei were stained with DAPI.

Protocols

References

ab126583 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting us. I have had the chance to speak with the development group as requested. AIF is an intracellular protein as such this particular assay (ab126583) has not been tested in serum yet. If there is good reason to believe that...

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