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Abcam’s Albumin Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of albumin levels in urine, saliva, milk, cerebrospinal fluids and cell culture samples.
An albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an albumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of albumin captured in plate.
|Components||1 x 96 tests|
|100X Streptavidin-Peroxidase Conjugate||1 x 80µl|
|10X Diluent N Concentrate||1 x 30ml|
|20X Wash Buffer Concentrate||2 x 30ml|
|60X Biotinylated Human Albumin Antibody||1 x 100µl|
|Albumin Microplate (12 x 8 well strips)||1 unit|
|Albumin Standard||1 vial|
|Chromogen Substrate||1 x 8ml|
|Sealing Tapes||3 units|
|Stop Solution||1 x 12ml|
Our Abpromise guarantee covers the use of ab108788 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Concentration of albumin from differently diluted (in parenthesis) samples, demonstrating a linearity of 84-90% in CSF and 88-111% in milk (duplicates; +/- SD).
Albumin in HepG2 (diluted 1:50-200) and HeLa (undiluted) supernatants, heparin-treated plasma and serum (5e5-5e6), urine (100-500), milk (5000-30000), saliva (200-2000) was measured in duplicates (+/- SD).
Standard curve with background signal subtracted (duplicates; +/- SD).
Human albumin measured in various samples showing mass (microgram) per mL sample tested
Representative Standard Curve using ab108788