• Product nameHuman Albumin ELISA Kit
    See all Albumin kits
  • Detection methodColorimetric
  • Precision
    Sample n Mean SD CV%
    Overall 4%
    Sample n Mean SD CV%
    Overall 10%
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 1.5 ng/ml
  • Range
    6.25 ng/ml - 50 ng/ml
  • Recovery

    97 %

  • Assay time
    3h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Albumin Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of albumin levels in urine, saliva, milk, cerebrospinal fluids and cell culture samples.

    An albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an albumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of albumin captured in plate.

    Get results in 90 minutes with Human Serum Albumin ELISA Kit (ab179887) from our SimpleStep ELISA® range.

    Please note: For both the 10X Diluent N Concentrate and 20X Wash Buffer Concentrate, please ensure to mix well by inversion before use to avoid precipitation.


  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate


  • FunctionSerum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
  • Tissue specificityPlasma.
  • Involvement in diseaseDefects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.
  • Sequence similaritiesBelongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • Post-translational
    Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
    Glycated in diabetic patients.
    Phosphorylation sites are present in the extracelllular medium.
    Acetylated on Lys-223 by acetylsalicylic acid.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • alb
    • Albumin (32 AA)
    • Albumin (AA 34)
    • cell growth inhibiting protein 42
    • DKFZp779N1935
    • GIG20
    • GIG42
    • growth-inhibiting protein 20
    • OTTHUMP00000220436
    • OTTHUMP00000220438
    • OTTHUMP00000220439
    • PRO0883
    • PRO0903
    • PRO1341
    • PRO1708
    • PRO2044
    • PRO2619
    • PRO2675
    • Serum albumin
    • UNQ696/PRO1341
    see all
  • Database links

Associated products


Our Abpromise guarantee covers the use of ab108788 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human Albumin ELISA Kit images

  • Concentration of albumin from differently diluted (in parenthesis) samples, demonstrating a linearity of 84-90% in CSF and 88-111% in milk (duplicates; +/- SD).

  • Albumin in HepG2 (diluted 1:50-200) and HeLa (undiluted) supernatants, heparin-treated plasma and serum (5e5-5e6), urine (100-500), milk (5000-30000), saliva (200-2000) was measured in duplicates (+/- SD).

  • Standard curve with background signal subtracted (duplicates; +/- SD).

  • Human albumin measured in various samples showing mass (microgram) per mL sample tested

  • Representative Standard Curve using ab108788


References for Human Albumin ELISA Kit (ab108788)

This product has been referenced in:
  • Tangedal S  et al. Comparison of inflammatory markers in induced and spontaneous sputum in a cohort of COPD patients. Respir Res 15:138 (2014). Human . Read more (PubMed: 25398249) »

See 1 Publication for this product

Product Wall

Thank you for your inquiry. Ab108788 is validated for use with milk samples. The Diluent N does not contain BSA or casein but a BSA like protein. It does not interfere with human albumin measurement or cross react.

The albumin ELISA ab108788 is designed to be able to detect the relatively low amounts (compared to serum and plasma) of albumin in the sample types listed on the datasheet: urine, saliva, milk, cerebrospinal fluid and cell culture supernatants. It wil...

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The laboratory has confirmed that the "10X Diluent Concentrate N" does not contain BSA or casein. It contains other proteins that have no cross-reactivity with human albumin.

Yes, we added this diluent to our catalog: Diluent Concentrate (10x) ab172376.

While this kit as not been tested with tissue or cell lysates, in general, a lysis buffer containg a low concentration of triton-X-100 or SP-40 (less than 1 %) will not affect the assay.

The source of the kit ab108788 informed me that they have never tested this kit with horse serum. They have tested it with BSA and they did not see any significant cross-reactivity.

The antibodies used in this kit are optimized for use in kit only. These are not available separately. We have other antibodies available in our catalogue that can be used to develop the assay e.g. ab10241/ ab2406, ab117455

Abreview for Albumin Human ELISA kit

Excellent Excellent 5/5 (Ease of Use)

Sample: Human Cell (HepG2 cells)
Specification: HepG2 cells
Amount of Sample: 0.2ug albumin standard µg
Type of Kit Used: ELISA
Positive control: Provided Albumin Standard
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: Recognized human albumin. Did not recognize bovine albumin in FBS.

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Submitted Jul 03 2012