Overview

  • Product nameHuman Alpha 1 acid Glycoprotein ELISA Kit (AGP)
    See all Alpha 1 Acid Glycoprotein kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.9%
    Inter-assay
    Sample n Mean SD CV%
    Overall 9%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 3 ng/ml
  • Range
    3.125 ng/ml - 200 ng/ml
  • Recovery

    = 97 %

  • Assay time
    4h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Alpha 1 acid Glycoprotein (AGP) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of AGP levels in cell culture supernatant, saliva, milk, and urine.

    An Alpha 1 acid Glycoprotein specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Alpha 1 acid Glycoprotein specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Alpha 1 acid Glycoprotein captured in plate.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionAppears to function in modulating the activity of the immune system during the acute-phase reaction.
  • Tissue specificityExpressed by the liver and secreted in plasma.
  • Sequence similaritiesBelongs to the calycin superfamily. Lipocalin family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • A1AG1_HUMAN
    • AGP
    • AGP 1
    • AGP A
    • AGP1
    • Alpha 1 acid glycoprotein
    • Alpha-1-acid glycoprotein 1
    • Epididymis secretory sperm binding protein Li 153w
    • HEL S 153w
    • OMD 1
    • ORM
    • ORM1
    • Orosomucoid 1
    • Orosomucoid-1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Human Alpha 1 acid Glycoprotein ELISA Kit (AGP) images

  • Representative Standard Curve using ab108852.

Protocols

References for Human Alpha 1 acid Glycoprotein ELISA Kit (AGP) (ab108852)

This product has been referenced in:

See 1 Publication for this product

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Thank you for your inquiry.

I can recommend ab108854 for your customer since this kit is tested and guaranteed for plasma and serum samples. ab108852 is not.

Please find the protocol booklet for ab108854 attached.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"