Overview

  • Product name
    Human Alpha 1 acid Glycoprotein ELISA Kit (AGP)
    See all Alpha 1 Acid Glycoprotein kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 5%
    Inter-assay
    Sample n Mean SD CV%
    Overall 9.7%
  • Sample type
    Saliva, Milk, Serum, Plasma
  • Assay type
    Competitive
  • Sensitivity
    = 0.1 µg/ml
  • Range
    0.063 µg/ml - 4 µg/ml
  • Recovery

    = 98.5 %

  • Assay time
    3h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Alpha 1 acid Glycoprotein (AGP) Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitive measurement of Alpha 1 acid Glycoprotein in plasma and serum.

    An Alpha 1 acid Glycoprotein specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Alpha 1 acid Glycoprotein is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Alpha 1 acid Glycoprotein captured in plate.

  • Tested applications
    Suitable for: Competitive ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108854 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent dilution.

Images

  • Alpha1 acid Glycoprotein measured in various biological fluids showing quantity (ng) per mL of sample tested

  • Standard curve : mean of duplicates (+/-SD)

Protocols

References

This product has been referenced in:
  • Palmer AC  et al. Provitamin A-biofortified maize increases serum ß-carotene, but not retinol, in marginally nourished children: a cluster-randomized trial in rural Zambia. Am J Clin Nutr 104:181-90 (2016). Competitive ELISA ; Human . Read more (PubMed: 27169838) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for your inquiry.

Unfortunately, we do not have any Human serum available in our catalog that could be used as control and the kit ab108854 (http://www.abcam.com/ab108854) comes with Human AGP Standard but not with control serum.
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Thank you for your reply.

We will update the graph, as it indeed confusing. When +2, 1, 0, -1 and -2is written, it means actually 102, 101, 100, 10-1, 10-2 so 100, 10, 1, 0.1 and 0.01.

Please excuse for this error. As said, I alread...

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Thank you for contacting us and your patience while we were investigating this issue.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product tha...

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Thank you for your inquiry.

I can recommend ab108854 for your customer since this kit is tested and guaranteed for plasma and serum samples. ab108852 is not.

Please find the protocol booklet for ab108854 attached.

Plasma a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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