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|Sample type||Average %||Range|
|Serum||> 85||% - %|
Abcam's alpha 1 Antichymotrypsin Human ELISA Kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of alpha 1 Antichymotrypsin levels in Human serum and plasma.
In this assay the alpha 1 Antichymotrypsin present in samples reacts with the anti-alpha 1 Antichymotrypsin antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-alpha 1 Antichymotrypsin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound alpha 1 Antichymotrypsin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of alpha 1 Antichymotrypsin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of alpha 1 Antichymotrypsin in the test sample. The quantity of alpha 1 Antichymotrypsin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Get results in 90 minutes with Human Alpha 1-antichymotrypsin ELISA Kit (ab171574) from our SimpleStep ELISA® range.
|Components||1 x 96 tests|
|100X HRP-conjugated anti-Human alpha 1 Antichymotrypsin antibody||1 x 150µl|
|20X Wash Buffer Concentrate||1 x 50ml|
|5X Diluent Concentrate||1 x 50ml|
|Chromogen Substrate Solution||1 x 12ml|
|Human alpha 1 Antichymotrypsin Calibrator (lyophilized)||1 vial|
|Human alpha 1 Antichymotrypsin ELISA Microplate||1 unit|
|Stop Solution||1 x 12ml|
Our Abpromise guarantee covers the use of ab157706 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab157706 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"