Overview

  • Product name
    Human alpha 1 Antichymotrypsin ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 10%
  • Sample type
    Serum, Plasma, Other biological fluids
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    1.722 ng/ml
  • Range
    6.25 ng/ml - 400 ng/ml
  • Recovery

    > 85 %

    Sample specific recovery
    Sample type Average % Range
    Serum > 85 % - %

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam's alpha 1 Antichymotrypsin Human ELISA Kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of alpha 1 Antichymotrypsin levels in Human serum and plasma.


    In this assay the alpha 1 Antichymotrypsin present in samples reacts with the anti-alpha 1 Antichymotrypsin antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-alpha 1 Antichymotrypsin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound alpha 1 Antichymotrypsin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromo­genic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of alpha 1 Antichymotrypsin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of alpha 1 Antichymotrypsin in the test sample. The quantity of alpha 1 Antichymotrypsin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.


    Get results in 90 minutes with Human Alpha 1-antichymotrypsin ELISA Kit (ab171574) from our SimpleStep ELISA® range.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab157706 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab157706 alpha 1 Antichymotrypsin Human ELISA Kit.

Protocols

References

ab157706 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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