• Product nameHuman alpha 1 Antitrypsin ELISA Kit (SERPINA1)
    See all alpha 1 Antitrypsin kits
  • Detection methodColorimetric
  • Precision
    Sample n Mean SD CV%
    Overall 3.7%
    Sample n Mean SD CV%
    Overall 8.6%
  • Sample type
    Saliva, Milk, Serum, Plasma, Cell Lysate
  • Assay typeCompetitive
  • Sensitivity
    = 0.018 µg/ml
  • Range
    0.039 µg/ml - 40 µg/ml
  • Recovery

    98 %

  • Assay time
    3h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s alpha 1 Antitrypsin (SERPINA1) Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of alpha 1 antitrypsin levels in plasma and serum.

    An alpha 1 antitrypsin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated alpha 1 Antitrypsin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of alpha 1 antitrypsin captured in plate.

    Get results in 90 minutes with Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (ab189579) from our SimpleStep ELISA® range.

  • Tested applicationsSuitable for: Competitive ELISAmore details
  • PlatformMicroplate


  • FunctionInhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
    Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
  • Tissue specificityUbiquitous. Expressed in leukocytes and plasma.
  • Involvement in diseaseAlpha-1-antitrypsin deficiency
  • Sequence similaritiesBelongs to the serpin family.
  • DomainThe reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.
  • Post-translational
    N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.
    Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418.
  • Cellular localizationSecreted. Endoplasmic reticulum. The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum and Secreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • Alternative names
    • A1A
    • A1AT
    • A1AT_HUMAN
    • AAT
    • Alpha 1 antiproteinase
    • Alpha 1 antitrypsin
    • Alpha 1 antitrypsin null
    • Alpha 1 protease inhibitor
    • Alpha-1 protease inhibitor
    • Alpha-1-antiproteinase
    • alpha1 proteinase inhibitor
    • Alpha1AT
    • Dom1
    • PI
    • PI1
    • PRO2275
    • Serine (or cysteine) proteinase inhibitor clade A member 1
    • Serine protease inhibitor 1-1
    • Serine protease inhibitor A1a
    • Serpin A1
    • Serpin A1a
    • Serpin peptidase inhibitor clade A member 1
    • Serpina1
    • Short peptide from AAT
    • SPAAT
    • Spi1-1
    see all
  • Database links

Associated products


Our Abpromise guarantee covers the use of ab108798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) images

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted
  • Alpha 1 Antitrypsin measured in biological fluids showing quantity (μg) per mL of tested sample. Serum and plasma samples diluted 100-8100 fold. Human milk and saliva diluted 10 fold. Hu urine diluted 1000 fold.


References for Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (ab108798)

This product has been referenced in:
  • Abdullah MI  et al. Tissue and serum samples of patients with papillary thyroid cancer with and without benign background demonstrate different altered expression of proteins. PeerJ 4:e2450 (2016). Competitive ELISA ; Human . Read more (PubMed: 27672505) »

See 1 Publication for this product

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ab108798 is for plasma and serum samples.

ab108799 is for urine, milk, saliva and cell culture samples

Unfortunately, neither of them has cross-reactivity with mouse
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You can use ab108799 for serum, you will just have to optimize the dilutions to get on the standard curve. Our serum samples were at 6000X.

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We unfortunately have not tested these products with sputum samples. Theoretically, these products will be OK however we cannot guarantee as we do not have tested data. The best way for using these products is by i...

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Could you please confirm and explain why the streptavidin conjugate reagent is insufficient for 96 wells plate? Is there any shortage in the volume provided or the reagent does not work properly?...

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