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Abcam’s alpha 1 Antitrypsin (SERPINA1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of alpha 1 Antitrypsin in urine, milk, saliva, CSF and cell culture supernatants.
An alpha 1 antitrypsin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an alpha 1 antitrypsin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of alpha 1 antitrypsin captured in plate.
Get results in 90 minutes with Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (ab189579) from our SimpleStep ELISA® range.
|Components||1 x 96 tests|
|100X Streptavidin-Peroxidase Conjugate||1 x 80µl|
|10X Diluent N Concentrate||1 x 30ml|
|20X Wash Buffer Concentrate||2 x 30ml|
|80X Biotinylated Human alpha 1 Antitrypsin Antibody||1 x 75µl|
|alpha 1 Antitrypsin Microplate (12 x 8 well strips)||1 unit|
|alpha 1 Antitrypsin Standard||1 vial|
|Chromogen Substrate||1 x 8ml|
|Sealing Tapes||3 units|
|Stop Solution||1 x 12ml|
Our Abpromise guarantee covers the use of ab108799 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Linearity: alpha 1 antitrypsin in urine at different diutions, corrected for the factor of dilution (duplicates +/- SD).
Linearity: alpha 1 antitrypsin in human milk at different diutions, corrected for the factor of dilution (duplicates +/- SD)
Linearity: alpha 1 antitrypsin in serum and plasma (Li-heparine) at different diutions, corrected for the factor of dilution (duplicates +/- SD)
Alpha 1 antitrypsin in human biological fluids (duplicates +/- SD).
The samples were tested in different ranges of dilutions (serum and Li-heparine plasma: 2e3-1e6; CSF: 5e3-5e4; milk: 1e2-1e3; saliva: 2e2-2e3).
Standard curve with background signal subtracted (duplicates; +/- SD).
Representative Standard Curve using ab108799