Human Alpha 1 microglobulin full length protein (ab96148)

Overview

Description

  • NatureNatural
  • SourceNative
  • Amino Acid Sequence
    • AccessionP02760
    • SpeciesHuman
    • Molecular weight30 kDa
    • Amino acids20 to 203

Specifications

Our Abpromise guarantee covers the use of ab96148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Radial Immunodiffusion

    SDS-PAGE

  • FormLyophilised
  • Additional NotesStarting material tested and found negative for HIV I & II antibodies, Hepatitis B surface antigen, and Hepatitis C antibodies.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Store at +4°C.

    Preservative: None
    Constituents: 0.02M Ammonium bicarbonate
    (May contain traces of buffer salts)

  • ReconstitutionReconstitution in phosphate buffer, pH >7.0 containing 0.15M NaCl is recommended.

General info

  • Alternative names
    • A1M
    • Alpha 1 microglobulin/bikunin precursor
    • Alpha 1 microglycoprotein
    • Alpha-1 microglycoprotein
    • Alpha-1-microglobulin
    • AMBP
    • AMBP_HUMAN
    • Bikunin
    • Complex-forming glycoprotein heterogeneous in charge
    • EDC1
    • Growth inhibiting protein 19
    • HCP
    • HI-30
    • HI30
    • IATIL
    • Inter-alpha-trypsin inhibitor light chain
    • ITI
    • ITI-LC
    • ITIL
    • Microglobulin (alpha 1)
    • Protein AMBP
    • Protein HC
    • Trypstatin
    • Uristatin
    • Uronic-acid-rich protein
    • UTI
    see all
  • FunctionInter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization.
    Trypstatin is a trypsin inhibitor.
  • Tissue specificityExpressed by the liver and secreted in plasma. Alpha-1-microglobulin occurs in many physiological fluids including plasma, urine, and cerebrospinal fluid. Inter-alpha-trypsin inhibitor is present in plasma and urine.
  • Sequence similaritiesIn the N-terminal section; belongs to the calycin superfamily. Lipocalin family.
    Contains 2 BPTI/Kunitz inhibitor domains.
  • Post-translational
    modifications
    The precursor is proteolytically processed into separately functioning proteins.
    3-hydroxykynurenine, an oxidized tryptophan metabolite that is common in biological fluids, reacts with Cys-53, Lys-111, Lys-137, and Lys-149 to form heterogeneous polycyclic chromophores including hydroxanthommatin. The reaction by alpha-1-microglobulin is autocatalytic; the human protein forms chromophore even when expressed in insect and bacterial cells. The chromophore can react with accessible cysteines forming non-reducible thioether cross-links with other molecules of alpha-1-microglobulin or with other proteins such as Ig alpha-1 chain C region 'Cys-352'.
    Heavy chains are interlinked with bikunin via a chondroitin 4-sulfate bridge to the their C-terminal aspartate.
    Addition of glycosaminoglycan chondroitin sulfate, allows cross-linking between the different components.
  • Cellular localizationSecreted.
  • Target information above from: UniProt accession P02760 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

References for Human Alpha 1 microglobulin full length protein (ab96148)

ab96148 has not yet been referenced specifically in any publications.

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