Overview

  • Product name
    Human alpha 2 Macroglobulin ELISA Kit
    See all alpha 2 Macroglobulin kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.4%
    Inter-assay
    Sample n Mean SD CV%
    Overall 8.1%
  • Sample type
    Cell culture supernatant, Saliva, Milk
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    = 2 ng/ml
  • Range
    0.002 µg/ml - 0.5 µg/ml
  • Recovery

    98 %

  • Assay time
    4h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s alpha 2 Macroglobulin Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of alpha 2 Macroglobulin in saliva, milk, cerebrospinal fluid and cell culture supernatants.

    An alpha 2 Macroglobulin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an alpha 2 Macroglobulin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of alpha 2 Macroglobulin captured in plate.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab108883.

Protocols

References

This product has been referenced in:
  • Sturtzel C  et al. The transcription factor MEF2C negatively controls angiogenic sprouting of endothelial cells depending on oxygen. PLoS One 9:e101521 (2014). Read more (PubMed: 24988463) »

See 1 Publication for this product

Customer reviews and Q&As



Unfortunately, I can confirm that the antibodies used in these kits have been raised against recombinant protein and purified proteins. The exact epitope of the antibodies have not been investigated further. We are therefore not able to provi...

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Abreview for alpha 2 Macroglobulin Human ELISA Kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews

Sample: Human Cell (HepG2)
Specification: HepG2
Amount of Sample: 0.125ug standard µg
Type of Kit Used: ELISA
Positive control: Provided alpha 2 Macroglobulin standard
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: Recognized human A2M. Did not recognize bovine A2M in FBS.
Username

Abcam user community

Verified customer

Submitted Jul 03 2012

Thank you for your inquiry. ab108884 is an alpha 1 microglobulin human ELISA kit, Swiss Prot P02760. Alpha1-microglobulin (1M), also called protein HC, is a tubular plasma and tissue protein that belongs to the lipocalin transport protein superfami...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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