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Abcam’s alpha Fetoprotein (AFP) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of alpha Fetoprotein in Human serum.
A 96-well plate has been precoated with anti-alpha Fetoprotein antibodies. Samples and standards and are added to the wells, where alpha Fetoprotein in the sample and standards binds to the precoated antibody. After incubation and washing, added Anti-alpha Fetoprotein-HRP conjugate binds to the antibody-alpha Fetoprotein complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of alpha Fetoprotein in the sample and the intensity is measured at 450 nm.
|Components||1 x 96 tests|
|alpha Fetoprotein Standard - 0 ng/mL||1 x 0.5ml|
|alpha Fetoprotein Standard - 150 ng/mL||1 x 0.5ml|
|alpha Fetoprotein Standard - 20 ng/mL||1 x 0.5ml|
|alpha Fetoprotein Standard - 300 ng/mL||1 x 0.5ml|
|alpha Fetoprotein Standard - 5 ng/mL||1 x 0.5ml|
|alpha Fetoprotein Standard - 50 ng/mL||1 x 0.5ml|
|Anti-alpha Fetoprotein Coated Microplate (12 x 8 wells)||1 unit|
|Anti-alpha Fetoprotein HRP Conjugate||1 x 18ml|
|Stop Solution||1 x 11ml|
|TMB Substrate Solution||1 x 11ml|
|Zero Buffer||1 x 13ml|
Our Abpromise guarantee covers the use of ab108631 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent dilution.|
ab108631 has not yet been referenced specifically in any publications.