Abcam’s alpha Fetoprotein Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of alpha Fetoprotein in serum and plasma.
A 96-well plate has been precoated with anti-alpha Fetoprotein IgG antibodies. Samples, standards and the alpha Fetoprotein-HRP conjugate are added to the wells, where alpha Fetoprotein in the sample and standards binds to the precoated antibody and added alpha Fetoprotein-HRP conjugate binds to this antibody-AFP complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of alpha Fetoprotein in the sample and the intensity is measured at 450 nm.
|Components||Identifier||1 x 96 tests|
|50X Washing Solution||1 x 20ml|
|alpha Fetoprotein Standard 0 – 0 ng/mL||1 x 1ml|
|alpha Fetoprotein Standard 1 – 5 ng/mL||1 x 1ml|
|alpha Fetoprotein Standard 2 – 20 ng/mL||1 x 1ml|
|alpha Fetoprotein Standard 3 – 80 ng/mL||1 x 1ml|
|alpha Fetoprotein Standard 4– 200 ng/mL||1 x 1ml|
|Anti-alpha Fetoprotein HRP Conjugate (Concentrated)||1 x 1ml|
|Anti-alpha Fetoprotein IgG Coated Microplate (12 x 8 wells)||12 strips of 8 wells||1 unit|
|Cover foil||1 unit|
|Incubation buffer||1 x 50ml|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|TMB Substrate Solution||1 x 15ml|
Our Abpromise guarantee covers the use of ab108680 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent dilution.|
ab108680 has not yet been referenced specifically in any publications.