Overview

  • Product name
    Human Amyloid beta 1-40 ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    0.1 ng/ml
  • Range
    0.1 ng/ml - 100 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 101.3 77% - 125%
    Plasma 97.39 68% - 136%
    Cell culture media 104.3 71% - 145%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Amyloid beta 1-40 Human ELISA Kit (ab193692) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Amyloid beta 1-40 in serum, plasma, and cell culture supernatants. (UniprotID: P05067)


    This assay employs an antibody specific for Human Amyloid beta 1-40 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures Amyloid beta 1-40 present in the samples. The wells are washed and biotinylated anti-Human Amyloid beta 1-40 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of Amyloid beta 1-40 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    1100X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent 1 x 15ml
    Biotinylated Human Amyloid beta detection antibody 2 vials
    Human Amyloid beta Standards 2 vials
    Pre-coated Human amyloid beta 1-40 microplate (12 strips x 8 wells) 1 unit
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml

Applications

Our Abpromise guarantee covers the use of ab193692 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human Amyloid beta 1-40 ELISA Kit images

  • Standard Curve using ab193692 Amyloid beta 1-40 Human ELISA Kit.

Protocols

References for Human Amyloid beta 1-40 ELISA Kit (ab193692)

ab193692 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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