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|Sample type||Average %||Range|
|Serum||101.3||77% - 125%|
|Plasma||97.39||68% - 136%|
|Cell culture media||104.3||71% - 145%|
Abcam’s Amyloid beta 1-40 Human ELISA Kit (ab193692) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Amyloid beta 1-40 in serum, plasma, and cell culture supernatants. (UniprotID: P05067)
This assay employs an antibody specific for Human Amyloid beta 1-40 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures Amyloid beta 1-40 present in the samples. The wells are washed and biotinylated anti-Human Amyloid beta 1-40 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of Amyloid beta 1-40 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
|Components||1 x 96 tests|
|1100X HRP-Streptavidin Concentrate||1 x 200µl|
|20X Wash Buffer Concentrate||1 x 25ml|
|5X Assay Diluent||1 x 15ml|
|Biotinylated Human Amyloid beta detection antibody (lyophilized)||2 vials|
|Human Amyloid beta Standards||2 vials|
|Pre-coated Human amyloid beta 1-40 microplate (12 strips x 8 wells)||1 unit|
|Stop Solution||1 x 8ml|
|TMB Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab193692 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab193692 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"