|Sample type||Average %||Range|
|Cell culture supernatant||95.25||83% - 105%|
|Serum||94.47||81% - 103%|
|Plasma||93.93||80% - 102%|
Abcam’s Angiopoietin 1 (ANG1) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Angiopoietin 1 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human Angiopoietin 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Angiopoietin 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Angiopoietin 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Angiopoietin 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimisation may be required with urine samples.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|400X HRP-Streptavidin Concentrate||1 x 200µl|
|5X Assay Diluent B||1 x 15ml|
|Angiopoietin 1 Microplate (12 x 8 well strips)||1 unit|
|Assay Diluent A||1 x 30ml|
|Biotinylated anti-Human Angiopoietin 1 (lyophilized)||2 vials|
|Recombinant Angiopoietin 1 Standard Human||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab99972 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|