• Product name
    Human Angiopoietin 2 ELISA Kit (ANG2)
    See all Angiopoietin 2 kits
  • Detection method
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 10 pg/ml
  • Range
    4.12 pg/ml - 3000 pg/ml
  • Recovery

    88 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 82.1 76% - 92%
    Serum 85.3 82% - 109%
    Plasma 96.9 95% - 116%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Angiopoietin 2 (ANG2) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Angiopoietin 2 in serum, plasma and cell culture supernates.

    This assay employs an antibody specific for Human Angiopoietin 2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Angiopoietin 2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Angiopoietin 2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Angiopoietin 2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimisation may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Function
    Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.
  • Sequence similarities
    Contains 1 fibrinogen C-terminal domain.
  • Domain
    The Fibrinogen C-terminal domain mediates interaction with the TEK/TIE2 receptor.
  • Cellular localization
  • Information by UniProt
  • Alternative names
    • AGPT2
    • ANG 2
    • ANG-2
    • ANG2
    • Angiopoietin 2a
    • Angiopoietin 2B
    • Angiopoietin-2
    • Angiopoietin2
    • ANGPT 2
    • Angpt2
    • Tie2 ligand
    see all
  • Database links


Our Abpromise guarantee covers the use of ab99971 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human Angiopoietin 2 ELISA Kit (ANG2) images

  • Representative Standard Curve using ab99971.


References for Human Angiopoietin 2 ELISA Kit (ANG2) (ab99971)

This product has been referenced in:
  • Zhou L  et al. Plasma angiopoietin-2 is persistently elevated after non-small cell lung cancer surgery and stimulates angiogenesis in vitro. Medicine (Baltimore) 95:e4493 (2016). Human . Read more (PubMed: 27512865) »
  • Genre F  et al. Antitumour necrosis factor-a therapy modulates angiopoietin-2 serum levels in non-diabetic ankylosing spondylitis patients. Ann Rheum Dis 72:1265-7 (2013). Read more (PubMed: 23482472) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab99971.
Please use the links above to contact us or submit feedback about this product.


Sign up