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|Sample type||Average %||Range|
|Cell culture supernatant||95.43||83% - 104%|
|Serum||92.45||81% - 101%|
|Plasma||93.31||82% - 102%|
Abcam’s Angiostatin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Angiostatin in serum, plasma (heparin) and cell culture supernatants.
This assay employs an antibody specific for Human Angiostatin coated on a 96-well plate. Standards and samples are pipetted into the wells and Angiostatin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Angiostatin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Angiostatin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimisation may be required with urine samples.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|5X Assay Diluent||1 x 15ml|
|800X HRP-Streptavidin Concentrate||1 x 200µl|
|Angiostatin Microplate (12 strips x 8 wells)||1 unit|
|Biotinylated anti-Human Angiostatin (lyophilized)||2 vials|
|Recombinant Human Angiostatin Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab99973 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Titration of NIBSC Plasmin (97/536), with background signal subtracted (duplicates; +/- SD).
Angiostatin measured in human undiluted biological fluids (duplicates +/- SD). Signals from mouse and rat (pooled) plasma and sera were below level of detection.
Standard curve with background signal subtracted (duplicates; +/- SD).
Representative Standard Curve using ab99973.
ab99973 has not yet been referenced specifically in any publications.