• Product nameHuman Angiotensin II ELISA Kit
  • Detection methodColorimetric
  • Precision
    Sample n Mean SD CV%
    Overall 5.2%
    Sample n Mean SD CV%
    Overall 8.8%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 0.06 ng/ml
  • Range
    0.063 ng/ml - 1 ng/ml
  • Assay time
    5h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Angiotensin II Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of angiotensin II in plasma, serum, and cell culture supernatants.

    An angiotensin II specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an angiotensin II specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of angiotensin II captured in plate.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate


  • FunctionEssential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis.
    Angiotensin-2: acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone.
    Angiotensin-3: stimulates aldosterone release.
    Angiotensin 1-7: is a ligand for the G-protein coupled receptor MAS1 (By similarity). Has vasodilator and antidiuretic effects (By similarity). Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets.
  • Tissue specificityExpressed by the liver and secreted in plasma.
  • Involvement in diseaseEssential hypertension
    Renal tubular dysgenesis
  • Sequence similaritiesBelongs to the serpin family.
  • Post-translational
    Beta-decarboxylation of Asp-34 in angiotensin-2, by mononuclear leukocytes produces alanine. The resulting peptide form, angiotensin-A, has the same affinity for the AT1 receptor as angiotensin-2, but a higher affinity for the AT2 receptor.
    In response to low blood pressure, the enzyme renin/REN cleaves angiotensinogen to produce angiotensin-1. Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2. Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3, angiotensin-4. Angiotensin 1-9 is cleaved from angiotensin-1 by ACE2 and can be further processed by ACE to produce angiotensin 1-7, angiotensin 1-5 and angiotensin 1-4. Angiotensin 1-7 has also been proposed to be cleaved from angiotensin-2 by ACE2 or from angiotensin-1 by MME (neprilysin).
    The disulfide bond is labile. Angiotensinogen is present in the circulation in a near 40:60 ratio with the oxidized disulfide-bonded form, which preferentially interacts with receptor-bound renin.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • AGT
    • Ang I
    • Ang II
    • Ang III
    • Ang IV
    • Angiotensin 1-10
    • Angiotensin 1-4
    • Angiotensin 1-8
    • Angiotensin 2
    • Angiotensin 2-8
    • Angiotensin 3-8
    • Angiotensin I
    • Angiotensin II
    • Angiotensin III
    • Angiotensin IV
    • Angiotensinogen
    • ANHU
    • Des-Asp[1]-angiotensin II
    • Serpin A8
    • Serpin peptidase inhibitor clade A member 8
    • SERPINA8
    see all
  • Database links


Our Abpromise guarantee covers the use of ab108796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human Angiotensin II ELISA Kit images

  • hu Angiotensin II measured in biological fluids showing quantity (pg) per mL of tested sample

  • Standard curve : mean of duplicates (+/-SD) with background readings subtracted


References for Human Angiotensin II ELISA Kit (ab108796)

This product has been referenced in:
  • Lu X  et al. Response of various conduit arteries in tachycardia- and volume overload-induced heart failure. PLoS One 9:e101645 (2014). Read more (PubMed: 25127035) »

See 1 Publication for this product

Product Wall

We have tested pig samples with this kit and noticed significant cross-reactivity. So pig samples should work with this kit.

ab108796 did not work for rat samples when tested in the laboratory and thus is not recommended for use with rat samples.

Abreview for Angiotensin II Human ELISA kit

Average Average 3/5 (Ease of Use)

Sample: Human Serum (Blood serum)
Specification: Blood serum
Amount of Sample: 10 µg
Type of Kit Used: ELISA
Positive control: QC control
Did you use the Abcam Kit protocol?: Yes

Abcam user community

Verified customer

Submitted Apr 29 2013

Thank you for your enquiry.

The lab replied with the following information:

Usually we use a 4-parameter trendline, but if that option isn’t available then a polynomial one will do fine. The factor 4 or 5 polynomia...

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Thank you for your patience in this matter.

I have talked to the lab and they have told me that you need to adjust pH to be able to dissolve EDTA. Probably PH 8.3-8.5 will work well. Check EDTA manufacturer datasheet to make sure.


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