Overview

  • Product name
    Human ANGPT4 ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    0.25 ng/ml
  • Range
    0.25 ng/ml - 200 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 101.4 88% - 120%
    Plasma 83.13 67% - 91%
    Cell culture media 113.7 95% - 130%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s ANGPT4 Human ELISA Kit (ab193693) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human ANGPT4 in serum plasma and cell culture supernatants.

    This assay employs an antibody specific for human ANGPT4 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures ANGPT4 present in the samples. The wells are washed and biotinylated anti-human ANGPT4 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of ANGPT4 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    600X HRP-Streptavidin Concentrate 1 x 200µl
    Assay Diluent A 1 x 30ml
    Biotinylated Human Angiopoietin-4 detection antibody (lyophilized) 2 vials
    Human Angiopoietin-4 Standards 2 vials
    Pre-coated Human Angiopoietin-4 Microplate (12 strips x 8 wells) 1 unit
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Function
    Binds to TEK/TIE2, modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2. Promotes endothelial cell survival, migration and angiogenesis.
  • Tissue specificity
    Highly expressed in the lung with much lower levels found in other tissues.
  • Sequence similarities
    Contains 1 fibrinogen C-terminal domain.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • ANG-3
    • ANG-4
    • Angiopoietin-3
    • Angiopoietin-4
    • ANGP4_HUMAN
    • ANGPT4
    • dJ824F16.2
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab193693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Standard Curve in Assay Diluent A using ab193693 ANGPT4 Human ELISA Kit.

    Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

  • Standard Curve in Assay Diluent B using ab193693 ANGPT4 Human ELISA Kit.

    Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Protocols

References

ab193693 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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