The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions. - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer. - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent. - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised. - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
Annexin II, heavy chain
Calpactin I heavy chain
Calpactin I heavy polypeptide
calpactin I heavy polypeptide (p36)
Calpactin-1 heavy chain
Epididymis secretory protein Li 270
HEL S 270
Placental anticoagulant protein IV
FunctionCalcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response.
Sequence similaritiesBelongs to the annexin family. Contains 4 annexin repeats.
DomainA pair of annexin repeats may form one binding site for calcium and phospholipid.
Post-translational modificationsPhosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface. ISGylated.
Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane. Melanosome. In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism.