Overview

  • Product name
    Human anti-Annexin V ELISA Kit
    See all Annexin V kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 2%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3.8%
  • Sample type
    Cell culture supernatant
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    1.2 ng/ml
  • Range
    6.3 ng/ml - 400 ng/ml
  • Recovery

    86 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 86 74% - 100%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s anti-Annexin V Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human anti-Annexin V antibody concentrations in cell culture supernatant.

    A 96-well plate has been precoated with Annexin V to which cognate antibodies bind. Standards or test samples are added to the wells and incubated. A horseradish peroxidase (HRP) labeled Annexin V conjugate is added to the wells, which is bound by the immobilized Annexin V-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of anti-Annexin V antibodies sample captured in the plate.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab119504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab119504.

Protocols

References

ab119504 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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