|Sample type||Average %||Range|
|Cell culture supernatant||86||74% - 100%|
Abcam’s anti-Annexin V Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human anti-Annexin V antibody concentrations in cell culture supernatant.
A 96-well plate has been precoated with Annexin V to which cognate antibodies bind. Standards or test samples are added to the wells and incubated. A horseradish peroxidase (HRP) labeled Annexin V conjugate is added to the wells, which is bound by the immobilized Annexin V-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of anti-Annexin V antibodies sample captured in the plate.
|Components||1 x 96 tests|
|20X Assay Buffer Concentrate||1 x 5ml|
|20X Wash Buffer Concentrate||1 x 50ml|
|Adhesive Films||2 units|
|HRP-Conjugate recombinant Annexin V||1 x 6ml|
|Human anti-Annexin V Standard lyophilized||2 vials|
|Human recombinant Annexin V coated microplate (12 x 8 wells)||1 unit|
|Stop Solution (1M Phosphoric acid)||1 x 15ml|
|TMB Substrate Solution||1 x 15ml|
Our Abpromise guarantee covers the use of ab119504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab119504 has not yet been referenced specifically in any publications.