• Product name
    Human Antithrombin III ELISA Kit
    See all Antithrombin III kits
  • Detection method
  • Precision
    Sample n Mean SD CV%
    Overall 4.8%
    Sample n Mean SD CV%
    Overall 7.1%
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture media, Cerebral Spinal Fluid
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    = 3 ng/ml
  • Range
    3.13 ng/ml - 200 ng/ml
  • Recovery

    98.5 %

  • Assay time
    4h 0m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Antithrombin III Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Antithrombin III in urine, plasma, milk, saliva, cerebrospinal fluid, serum, and cell culture supernatants.

    An antithrombin III specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an antithrombin III specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of antithrombin III captured in plate.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Function
    Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.
  • Tissue specificity
    Found in plasma.
  • Involvement in disease
    Antithrombin III deficiency
  • Sequence similarities
    Belongs to the serpin family.
  • Post-translational
    Phosphorylation sites are present in the extracellular medium.
  • Cellular localization
    Secreted > extracellular space.
  • Information by UniProt
  • Alternative names
    • ANT3_HUMAN
    • Antithrombin-III
    • ATIII
    • Serpin C1
    • SERPINC1
    see all
  • Database links


Our Abpromise guarantee covers the use of ab108801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human Antithrombin III ELISA Kit images

  • Linearity: Antithrombin III in samples at different diutions, corrected for the factor of dilution  (duplicates +/- SD)

  • Linearity: Antithrombin III in samples at different diutions, corrected for the factor of dilution (duplicates +/- SD), showing ng x mL-1 on y-axis.

  • Linearity: Antithrombin III in samples at different diutions, corrected for the factor of dilution  (duplicates +/- SD)

  • Antithrombin III in cell culture supernatants (duplicates +/- SD), tested in the dilution range of 1:1-1:100.

  • Antithrombin III in human biological fluids (duplicates +/- SD). The samples were differently diluted  (serum and Li-heparine plasma: 1e4-1e5; CSF: 1e2-1e3; milk: 10-100; saliva: 5-100; urine: neat)

  • Representative standard curve using ab108801.


References for Human Antithrombin III ELISA Kit (ab108801)

ab108801 has not yet been referenced specifically in any publications.

Product Wall

Please find below the information on how to prepare the standard for lots GR116626-1, Lot GR115289-1 and Lot GR106377-1 of the Antithrombin III Human ELISA Kit ab108801:

· MIX Diluent Concentrate (10x): If crystals have formed in t...

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