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|Sample type||Average %||Range|
|Serum||115||111% - 122%|
|Tissue Culture Media||89||85% - 92%|
|Heparin Plasma||95||76% - 111%|
|EDTA Plasma||112||108% - 115%|
|Citrate Plasma||119||117% - 123%|
Abcam’s Apolipoprotein AI (APOA1) in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Apolipoprotein AI protein in human serum, plasma and cell culture supernatants.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Apolipoprotein AI (ApoA1) is secreted by the liver and small intestine and is a major protein of plasma HDL (high density lipoprotein). APOA-I participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). Defects in APOA1I are associated with several diseases associated with low HDL levels (HDLD1 and HDLD2) and amyloidosis (AMYL8).
|Components||1 x 96 tests|
|10X APOA1 Capture Antibody||1 x 600µl|
|10X APOA1 Detector Antibody||1 x 600µl|
|10X Wash Buffer PT (ab206977)||1 x 20ml|
|Antibody Diluent 5BI||1 x 6ml|
|Human APOA1 Lyophilized Recombinant Protein||2 vials|
|Plate Seals||1 unit|
|Sample Diluent NS (ab193972)||1 x 50ml|
|SimpleStep Pre-Coated 96-Well Microplate (ab206978)||1 unit|
|Stop Solution||1 x 12ml|
|TMB Substrate||1 x 12ml|
Our Abpromise guarantee covers the use of ab189576 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Background-subtracted data values (mean +/- SD) are graphed.
Human serum and plasma (citrate) diluted 2.5x105 –fold to 8x106 –fold in Sample Diluent NS. Background subtracted data from duplicate measurements are plotted.
Interpolated concentrations of Apolipoprotein A1 in Human serum and plasma heparin. The concentrations of Apolipoprotein A1 were measured in duplicate and interpolated from the Apolipoprotein A1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apolipoprotein A1 concentration was determined to be 2500 µg/mL in serum and 1053ug/mL in plasma heparin.
Interpolated concentrations of Apolipoprotein A1 in Human serum from 10 donors. Serum from 10 apparently healthy male donors was measured in duplicate. The mean Apolipoprotein A1 concentration was determined to be 526 µg/mL with a range of 9.8-2011 µg/mL.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"