Human Apolipoprotein AI ELISA Kit (APOA1) (ab108803)

Overview

  • Product name
    Human Apolipoprotein AI ELISA Kit (APOA1)
    See all Apolipoprotein A I kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4%
    Inter-assay
    Sample n Mean SD CV%
    Overall 10.1%
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Cerebral Spinal Fluid
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    = 3 ng/ml
  • Range
    6.25 ng/ml - 100 ng/ml
  • Recovery

    97 %

  • Assay time
    4h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Apolipoprotein AI (APOA1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of apolipoprotein AI concentrations in plasma, serum, urine, saliva, milk, CSF and cell culture samples.


    An Apolipoprotein AI specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Apolipoprotein AI specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Apolipoprotein AI captured in plate.


    Get results in 90 minutes with Human Apolipoprotein AI ELISA Kit (ab189576) from our SimpleStep ELISA® range.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Human Apolipoprotein AI Antibody 1 x 120µl
    Apolipoprotein AI Microplate (12 x 8 well strips) 1 unit
    Chromogen Substrate 1 x 8ml
    Human Apolipoprotein AI Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • Research areas
  • Function
    Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
  • Tissue specificity
    Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b genotype and exposure to cigarette smoking. It is also present in increased levels in aortic lesions relative to native ApoA-I and increased levels are seen with increasing severity of disease.
  • Involvement in disease
    High density lipoprotein deficiency 2
    High density lipoprotein deficiency 1
    APOA1 mutations may be involved in the pathogenesis of amyloid polyneuropathy-nephropathy Iowa type, also known as amyloidosis van Allen type or familial amyloid polyneuropathy type III (PubMed:3142462 and PubMed:2123470). The clinical picture is dominated by neuropathy in the early stages of the disease and nephropathy late in the course. Death is due in most cases to renal amyloidosis.
    Amyloidosis 8
  • Sequence similarities
    Belongs to the apolipoprotein A1/A4/E family.
  • Post-translational
    modifications
    Palmitoylated.
    Met-110 and Met-136 are oxidized to methionine sulfoxides.
    Phosphorylation sites are present in the extracellular medium.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • Apo-AI
    • ApoA-I
    • APOA1
    • APOA1_HUMAN
    • Apolipoprotein A-I
    • Apolipoprotein A-I(1-242)
    • Apolipoprotein A1
    • Truncated apolipoprotein A-I
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab108803 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • APOA1 measured in various samples showing quantity (microgram) per mL of tested sample

  • Representative Standard Curve using ab108803

Protocols

References

ab108803 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Measure APOA1 in plasma samples

Excellent Excellent 5/5 (Ease of Use)
Abreviews
I measured APOA1 in plasma sample using ab108803 which should measure in cell culture supernatant, saliva, milk, urine, or cerebral spinal fluid. I tried to find the dilution factor for plasma samples. I found that 1:50000 is the optimum ratio for dilution and detection. Ultimately, this product can measure APOA1 in plasma samples.
Username

Abcam user community

Verified customer

Submitted Dec 28 2016


The amount of standard for lot GR132237-1 = 3.2 ug (micrograms).

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The lab let me know that the kit did not work for Bovine samples.

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I can confirm that the main difference between these kits is that the ab108804 Apolipoprotein A1 Human ELISA Kit uses a competitive ELISA quantification, whereas ab108803 Apolipoprotein A I Human ELISA Kituses sta...

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I have also forwarded this information to the laboratory in order to investigate the problem together. The laboratory and I do agree that all steps have been performed as indicated and do not ...

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