Human Apolipoprotein AII ELISA Kit (APOA2) (ab108805)

Overview

  • Product nameHuman Apolipoprotein AII ELISA Kit (APOA2)
    See all Apolipoprotein AII kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.7%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.2%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Milk, Urine, Serum, Plasma, Cell culture extracts
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 0.004 µg/ml
  • Range
    0.004 µg/ml - 4 µg/ml
  • Recovery

    97 %

  • Assay time
    4h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Apolipoprotein AII (APOA2) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of apolipoprotein AII concentrations in cell culture samples, serum and plasma, urine and milk.


    An Apolipoprotein AII specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Apolipoprotein AII specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Apolipoprotein AII captured in plate.


    For higher reproducibility in only 90 minutes, we recommend our SimpleStep ELISA® range made with recombinant monoclonal antibodies. Try Human Apolipoprotein AII ELISA Kit (ab184859).

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceMay stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism.
  • Cellular localizationSecreted.
  • Alternative names
    • Apo-AII
    • Apolipoprotein A-II
    • Apolipoprotein A2
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab108805 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human Apolipoprotein AII ELISA Kit (APOA2) images

  • Representative Standard Curve using ab108805

Protocols

References for Human Apolipoprotein AII ELISA Kit (APOA2) (ab108805)

ab108805 has not yet been referenced specifically in any publications.

Product Wall

Abreviews

Sample: Human
Amount of Sample: 0-20 µg
Type of Kit Used: ELISA
Positive control: APOB, APOAII, HDL, LDL
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: Kit detects APOB
Abcam response: We thank the customer for this feedback and we are currently working to resolve this issue.
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Verified customer

Submitted May 04 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"