Human Apolipoprotein CI ELISA Kit (APOCI) (ab108808)

Overview

  • Product nameHuman Apolipoprotein CI ELISA Kit (APOCI)
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.6%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.1%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 0.06 µg/ml
  • Range
    0.063 µg/ml - 4 µg/ml
  • Recovery

    98.5 %

  • Assay time
    5h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Apolipoprotein CI (APOCI) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of apolipoprotein CI concentrations in plasma, serum, cell lysates and cell culture supernatants.

    An Apolipoprotein CI specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Apolipoprotein CI specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Apolipoprotein CI captured in plate.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionAppears to modulate the interaction of APOE with beta-migrating VLDL and inhibit binding of beta-VLDL to the LDL receptor-related protein. Binds free fatty acids and reduces their intracellular esterification.
  • Tissue specificitySynthesized mainly in liver and to a minor degree in intestine. Secreted in plasma.
  • Sequence similaritiesBelongs to the apolipoprotein C1 family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • Apo-CIB
    • Apo-CIB''
    • ApoC-IB
    • ApoC-IB''
    • APOC1
    • APOC1_HUMAN
    • Apolipoprotein C1
    • Truncated apolipoprotein C-I
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108808 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human Apolipoprotein CI ELISA Kit (APOCI) images

  • Representative Standard Curve using ab108808

Protocols

References for Human Apolipoprotein CI ELISA Kit (APOCI) (ab108808)

ab108808 has not yet been referenced specifically in any publications.

Product Wall

Unfortunately I will not be able to send you a sample kit for testing purpose because this is against the company guidelines. All our product are vigorously tested in compatible samples and are fully guaranteed so if journal has any problem we can prov...

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I am sorry for the delay in getting back to you.

I can confirm that the antibodies used in this ELISA are polyclonal antibodies raised against the full length Apo CI protein.Theexact epitopes of binding are therefore not known and it is likel...

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Thank you for your call.

I can now confirm that in the kit with the lot number1239351 (amount is lotspecific)each vial contains 2 ug of Apo C-I standard.

We provide 2 vials of Apo C-I standard so the customer can use a fresh vial of...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"