Overview

  • Product nameHuman ARP4 ELISA Kit (Angiopoietin-Like 4)
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 20 pg/ml
  • Range
    27.4 pg/ml - 20000 pg/ml
  • Recovery

    100.4 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 117.8 67% - 137%
    Serum 95.38 85% - 104%
    Plasma 88.04 74% - 103%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s ARP4 (Angiopoietin-like 4) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human ARP4 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human ARP4 coated on a 96-well plate. Standards and samples are pipetted into the wells and ARP4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human ARP4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ARP4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimisation may be required with urine samples.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceARP4 is a fasting-induced adipose factor. It was identified as an adipocytokine up-regulated by fasting, by peroxisome proliferator-activated receptor agonists, and by hypoxia. At the protein level, in human and mouse blood plasma, ARP4 was found to be present both as a native protein and in a truncated form. Differentiation of mouse 3T3-L1 adipocytes was associated with the production of truncated ARP4, whereas in human white adipose tissue and SGBS adipocytes, only the native ARP4 could be detected. Interestingly, the truncated ARP4 was produced by human liver. Experimental data suggest that ARP4 is mainly presented in human blood plasma in a truncated form (ARP4-S2), whose level is increased by fenofibrate treatment. Levels of both truncated and native ARP4 showed marked inter individual variation but were not associated with body mass index and were not influenced by prolonged semistarvation.
  • Alternative names
    • 425O18 1
    • Angiopoietin like 4
    • Angiopoietin related protein 4
    • ANGPT L2
    • ANGPT L4
    • ANGPTL2
    • ANGPTL4
    • Fasting induced adipose factor
    • FIAF
    • Fibrinogen/angiopoietin related protein
    • Hepatic fibrinogen/angiopoietin related protein
    • HFARP
    • Major histocompatibility complex region NG27
    • NL2
    • Peroxisome proliferator-activated receptor (PPAR) gamma induced angiopoietin related protein
    • PGAR
    • pp1158
    • PPARG angiopoietin related protein
    • PSEC0166
    • Secreted protein Bk89
    • Weakly similar to angiopoietin 1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab99974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human ARP4 ELISA Kit (Angiopoietin-Like 4) images

  • Representative standard curve using ab99974.

  • Representative standard curve using ab99974.

Protocols

References for Human ARP4 ELISA Kit (Angiopoietin-Like 4) (ab99974)

This product has been referenced in:
  • Frenzel E  et al. a1-Antitrypsin Combines with Plasma Fatty Acids and Induces Angiopoietin-like Protein 4 Expression. J Immunol 195:3605-16 (2015). Read more (PubMed: 26363050) »
  • Zhao X  et al. Clostridium butyricum reduce lipogenesis through bacterial wall components and butyrate. Appl Microbiol Biotechnol 98:7549-57 (2014). Read more (PubMed: 24878750) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"