Abcam’s anti-Ascaris lumbricoides IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Ascaris lumbricoides in Human serum and plasma.
A 96-well plate has been precoated with Ascaris lumbricoides antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled Protein A conjugate is added to the wells, which binds to the immobilized Ascaris lumbricoides-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Ascaris lumbricoides IgG sample captured in plate.
|Components||Identifier||1 x 96 tests|
|20X Washing Solution||White cap||1 x 50ml|
|Ascaris lumbricoides (IgG) Coated Microplate (12 x 8 wells)||1 unit|
|Ascaris lumbricoides IgG Cut-off Control||Green cap||1 x 3ml|
|Ascaris lumbricoides IgG Negative Control||Blue cap||1 x 2ml|
|Ascaris lumbricoides IgG Positive Control||Red cap||1 x 2ml|
|Ascaris lumbricoides Protein A HRP Conjugate||Black cap||1 x 20ml|
|Cover foil||1 unit|
|IgG Sample Diluent||White cap||1 x 100ml|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|TMB Substrate Solution||Yellow cap||1 x 15ml|
Our Abpromise guarantee covers the use of ab108707 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent concentration.|
ab108707 has not yet been referenced specifically in any publications.