The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking - Blocking peptide for Anti-ATF-4 antibody (ab23760)
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions. - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer. - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent. - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised. - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
Activating transcription factor 4
cAMP-dependent transcription factor ATF-4
cAMP-responsive element-binding protein 2
Cyclic AMP dependent transcription factor ATF 4
Cyclic AMP response element binding protein 2
Cyclic AMP-dependent transcription factor ATF-4
Cyclic AMP-responsive element-binding protein 2
DNA binding protein TAXREB67
DNA-binding protein TAXREB67
Tax Responsive Enhancer Element B67
Tax-responsive enhancer element-binding protein 67
FunctionTranscriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I.
Sequence similaritiesBelongs to the bZIP family. Contains 1 bZIP domain.
Cellular localizationCytoplasm. Cell membrane. Nucleus. Colocalizes with GABBR1 in hippocampal neuron dendritic membranes.