Human Atherosclerosis Antibody Array - Membrane (20 targets) (ab169803)

Overview

  • Product name
    Human Atherosclerosis Antibody Array - Membrane (20 targets)
    See all Atherosclerosis Antibody Array antibody arrays
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay type
    Semi-quantitative
  • Species reactivity
    Reacts with: Human
  • Product overview

    ab169803 detects 20 Human Atherosclerotic Factors in 4 or 8 samples. Suitable for all liquid sample types.


    Targets: Eotaxin-1, G-CSF, GDNF, GM-CSF, ICAM-1, IFN-gamma, IL-1 alpha, IL-1 beta, IL-1 sRI, IL-1 sRII, MCP-1, M-CSF, TGF beta 1, TGF beta 2, TGF beta 3, TNF alpha, TNF beta, MIP-3 alpha, PDGF-BB, RANTES


    Learn more about membrane antibody arrays

  • Notes

    If you are interested in this cytokine array, arrays ab169817ab133998 and ab169809 may also be of interest. A list of human antibody membrane arrays is available here.

  • Tested applications
    Suitable for: Multiplex Protein Detectionmore details

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 4 Membranes 1 x 8 Membranes
    1,000X HRP-Streptavidin Buffer 1 x 50µl 1 x 50µl
    Blocking Buffer 1 x 25ml 1 x 50ml
    2000X Biotin-Conjugated Anti Cytokines 2 vials 4 vials
    20X Wash Buffer I 1 x 10ml 1 x 20ml
    20X Wash Buffer II 1 x 10ml 1 x 20ml
    2X Cell Lysis Buffer 1 x 10ml 1 x 20ml
    8-Well Incubation Tray (with Lid) 1 unit 1 unit
    Atherosclerosis Antibody Array Membranes 4 units 8 units
    Detection Buffer C 1 x 1.5ml 1 x 2.5ml
    Detection Buffer D 1 x 1.5ml 1 x 2.5ml
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab169803 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Multiplex Protein Detection Use at an assay dependent concentration.

Images

  • Typical results obtained with Abcam Membrane Antibody Arrays. Image above presents typical images obtained with Abcam Human Cytokine Membrane Antibody Array. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at RT for 1 min.

Protocols

References

ab169803 has not yet been referenced specifically in any publications.

Customer reviews and Q&As




According to the protocol, this "Antibody arrays provide high-content screening using the same sample volume as for ELISA"

The usual sample volume for an ELISA well is 200ul.

Following the the protocol, under 10. SAMPL...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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