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Abcam’s Human B7-1 (CD80) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human B7-1 in cell culture supernatants and serum.
A B7-1 specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for B7-1 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human B7-1 amount of sample captured in plate.
|Components||1 x 96 tests|
|ABC Diluent Buffer||1 x 12ml|
|Antibody Diluent Buffer||1 x 12ml|
|Anti-Human B7-1 Antibody Microplate (12 x 8 wells)||1 x 96 tests|
|Avidin-Biotin-Peroxidase Complex (ABC)||1 x 130µl|
|Biotinylated anti-Human B7-1 antibody||1 x 130µl|
|Lyophilized recombinant Human B7-1 standard||2 x 10ng|
|Plate Seal||4 units|
|Sample Diluent Buffer||1 x 30ml|
|TMB Color Developing Agent||1 x 10ml|
|TMB Stop Solution||1 x 10ml|
Our Abpromise guarantee covers the use of ab171342 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab171342 has not yet been referenced specifically in any publications.