Overview

  • Product name
    Human BAFF ELISA Kit (TNFSF13B)
    See all BAFF kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 2 pg/ml
  • Range
    62.5 pg/ml - 4000 pg/ml
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Human BAFF (TNFSF13B) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human BAFF (TNFSF13B) in cell culture supernatants, serum and plasma (heparin, EDTA).


    A BAFF (TNFSF13B) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for BAFF (TNFSF13B) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human BAFF (TNFSF13B) amount of sample captured in plate.


    Get higher sensitivity in only 90 minutes with Human BAFF ELISA Kit (TNFSF13B) (ab188391) from our SimpleStep ELISA® range.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Function
    Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B-and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.
    Isoform 2 seems to inhibit isoform 1 secretion and bioactivity.
  • Tissue specificity
    Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas. Isoform 2 is expressed in many myeloid cell lines.
  • Sequence similarities
    Belongs to the tumor necrosis factor family.
  • Post-translational
    modifications
    The soluble form derives from the membrane form by proteolytic processing.
    Isoform 2 is not efficiently shed from the membrane unlike isoform 1.
    N-glycosylated.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Alternative names
    • B lymphocyte stimulator
    • B-cell-activating factor
    • BAFF
    • BLyS
    • CD257
    • Dendritic cell-derived TNF-like molecule
    • soluble form
    • TALL-1
    • TN13B_HUMAN
    • TNF- and APOL-related leukocyte expressed ligand 1
    • TNFSF13B
    • Tumor necrosis factor ligand superfamily member 13b
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab119579 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human BAFF ELISA Kit (TNFSF13B) images

  • Representative Standard Curve using ab119579.

Protocols

References for Human BAFF ELISA Kit (TNFSF13B) (ab119579)

This product has been referenced in:
  • Mameli G  et al. Serum BAFF levels, Methypredsinolone therapy, Epstein-Barr Virus and Mycobacterium avium subsp. paratuberculosis infection in Multiple Sclerosis patients. Sci Rep 6:29268 (2016). Read more (PubMed: 27383531) »

See 1 Publication for this product

Product Wall



1.) Yes, a washing step is needed. This washing step is carried out when following the preparation of the plate under point 7 A.

This plate preparation should be done immediately before starting the assay procedure.

2.) No...

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The strips are divisible and precoated with antibodies, so you can use the wells directly.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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