Overview

  • Product name
    Human BCA1 ELISA Kit
    See all BCA1 kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 1.5 pg/ml
  • Range
    1.37 pg/ml - 1000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95.62 84% - 105%
    Serum 95.79 82% - 105%
    Plasma 93.68 83% - 104%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s BCA1 (BLC) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human BCA1 in serum, plasma, and cell culture supernatants.


    This assay employs an antibody specific for Human BCA1 coated on a 96-well plate. Standards and samples are pipetted into the wells and BCA1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human BCA1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BCA1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get results in 90 minutes with Human BCA1 ELISA Kit (CXCL13) (ab179881) from our SimpleStep ELISA® range.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200x HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    BCA1 Microplate (12 x 8 wells) 1 unit
    Biotinylated anti-Human BCA1 2 vials
    Recombinant Human BCA1 Standard 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • Research areas
  • Relevance
    B lymphocyte chemoattractant, independently cloned and named Angie, is a CXC chemokine strongly expressed in the follicles of the spleen, lymph nodes, and Peyer's patches. It preferentially promotes the migration of B lymphocytes (compared to T cells and macrophages), apparently by stimulating calcium influx into, and chemotaxis of, cells expressing Burkitt's lymphoma receptor 1 (BLR1). It may therefore function in the homing of B lymphocytes to follicles.
  • Alternative names
    • ANGIE
    • ANGIE2
    • B cell attracting chemokine 1
    • B cell chemoattractant
    • B cell homing chemokine (ligand for Burkitt's lymphoma receptor 1)
    • B lymphocyte chemoattractant
    • BCA 1
    • BLC
    • BLR1L
    • C X C motif chemokine 13
    • Chemokine (C X C motif) ligand 13
    • Chemokine (C X C motif) ligand 13 (B cell chemoattractant)
    • Chemokine CXC motif ligand 13
    • CXC chemokine BLC
    • CXCL13
    • SCYB13
    • Small inducible cytokine B subfamily (Cys X Cys motif) member 13 (B cell chemoattractant)
    • Small inducible cytokine B13
    • Small inducible cytokine subfamily B, member 13
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab99981 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab99981.

  • Representative Standard Curve using ab99981.

Protocols

References

ab99981 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab99981.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up