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|Sample type||Average %||Range|
|Serum||89||86% - 90%|
|Cell culture media||89||86% - 92%|
|Bovine Serum Albumin||85||82% - 88%|
Abcam’s Bcl-2 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Bcl-2 protein in human cell and tissue extracts.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Bcl-2 is the founding member of the apoptotic-regulating Bcl-2 family of proteins. It is considered an apoptosis suppressor in many cell systems and has been shown to interact with many other proteins involved in cell cycle regulation, autophagy, mitochondrial membrane stability, and oxidative stress response, among many others. As such an important protein involved in cell regulation, mutation or damage to the protein’s structure or function are critically linked to a variety of cancers. In particular, a chromosomal translocation occurs in follicular lymphoma which causes the Bcl-2 gene to relocate next to the heavy chain immunoglobulin locus. The unregulated expression of the fusion protein causes an excessively high amount of Bcl-2, disrupting the cell’s apoptosis regulation and leading to unregulated cell growth.
|Components||1 x 96 tests|
|10X Human Bcl-2 Capture Antibody||1 x 600µl|
|10X Human Bcl-2 Detector Antibody||1 x 600µl|
|10X Wash Buffer PT (ab206977)||1 x 20ml|
|50X Cell Extraction Enhancer Solution (ab193971)||1 x 1ml|
|5X Cell Extraction Buffer PTR (ab193970)||1 x 10ml|
|Antibody Diluent CPI||1 x 6ml|
|Human Bcl-2 Lyophilized Recombinant Protein||2 vials|
|Plate Seals||1 unit|
|Sample Diluent NS||1 x 12ml|
|SimpleStep Pre-Coated 96-Well Microplate (ab206978)||1 unit|
|Stop Solution||1 x 12ml|
|TMB Substrate||1 x 12ml|
Our Abpromise guarantee covers the use of ab202411 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Background-subtracted data values (mean +/- SD) are graphed.
Interpolated values were multiplied by their dilution factor to represent the level in Jurkat, HL-60, and SH-SY5Y cell extracts starting from undiluted concentrations of 125, 500, and 250 µg/mL, respectively.
Background-subtracted data values are graphed.
ab202411 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"