Human Bcl-2 protein fragment (ab79942)
- Product nameHuman Bcl-2 protein fragmentSee all Bcl-2 proteins and peptides ...
- SourceE. coli
- Amino Acid Sequence
- Amino acids1 to 211
Our Abpromise guarantee covers the use of ab79942 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- Purity> 90
% by SDS-PAGE.
- Concentration information loading...
Preparation and Storage
- Stability and Storage
Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 30% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8.0
- Apoptosis regulator Bcl 2
- Apoptosis regulator Bcl-2
- Apoptosis regulator Bcl2
- B cell CLL/lymphoma 2
- B cell leukemia/lymphoma 2
- Leukemia/lymphoma, B-cell, 2
- Oncogene B-cell leukemia 2
- Protein phosphatase 1, regulatory subunit 50
- FunctionSuppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).
- Tissue specificityExpressed in a variety of tissues.
- Involvement in diseaseA chromosomal aberration involving BCL2 has been found in chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
- Sequence similaritiesBelongs to the Bcl-2 family.
- DomainThe BH4 motif is required for anti-apoptotic activity and for interaction with RAF1 and EGLN3.
modificationsPhosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A).
Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.
Monoubiquitinated by PARK2, leading to increase its stability.
- Cellular localizationMitochondrion outer membrane. Nucleus membrane. Endoplasmic reticulum membrane.
References for Human Bcl-2 protein fragment (ab79942)
ab79942 has not yet been referenced specifically in any publications.