Overview

  • Product nameHuman BDNF ELISA Kit
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Milk, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 80 pg/ml
  • Range
    0.066 ng/ml - 16 ng/ml
  • Recovery

    93 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 93.35 83% - 103%
    Serum 94.57 83% - 103%
    Plasma 92.48 82% - 102%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Product overview

    Abcam’s BDNF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human BDNF in serum, plasma, and cell culture supernatants.


    This assay employs an antibody specific for Human BDNF coated on a 96-well plate. Standards and samples are pipetted into the wells and BDNF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human BDNF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BDNF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceBrain derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors that includes NGF, NT3, and NT4. All neurotrophins have six conserved cysteine residues and share a 55% sequence identity at the amino acid level. BDNF is a potent neurotrophic factor that supports the growth and survivability of nerve and/or glial cells. BDNF has been shown to enhance the survival and differentiation of several classes of neurons in vitro, including neural crest and placode derived sensory neurons, dopaminergic neurons in the substantia nigra, basal forebrain cholinergic neurons, hippocampal neurons, and retinal ganglial cells. BDNF is expressed within peripheral ganglia and is not restricted to neuronal target fields, raising the possibility that BDNF has paracrine or even autocrine actions on neurons as well as non neuronal cells. Expression of BDNF is reduced in both Alzheimer's and Huntington disease patients. In addition, functional studies showed that age-associated changes in BDNF-mediated pathways can enhance inflammation and increase myocardial injury after myocardial infarction in the aging heart.
  • Cellular localizationSecreted
  • Alternative names
    • Abrineurin
    • ANON2
    • Brain derived neurotrophic factor
    • BULN2
    • MGC34632
    • Neurotrophin
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab99978 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human BDNF ELISA Kit images

  • Human BDNF measured in cell culture supernatants showing quantity (pg) per mL of tested sample.

  • Human BDNF measured in biological fluids showing quantity (pg) per mL of tested sample. Hu plasma diluted 10-270 fold. Hu serum diluted 100-300 fold. Hu milk and saliva diluted 1-3 fold. Mouse and rat samples diluted 1-3 fold.

  • Representative Standard Curve using ab99978.

  • Representative Standard Curve using ab99978.

Protocols

References for Human BDNF ELISA Kit (ab99978)

This product has been referenced in:
  • Ramos-Cejudo J  et al. Brain-derived neurotrophic factor administration mediated oligodendrocyte differentiation and myelin formation in subcortical ischemic stroke. Stroke 46:221-8 (2015). Read more (PubMed: 25395417) »
  • Masvekar RR  et al. Morphine Enhances HIV-1SF162-Mediated Neuron Death and Delays Recovery of Injured Neurites. PLoS One 9:e100196 (2014). Read more (PubMed: 24949623) »

See all 2 Publications for this product

Product Wall

Detection of mouse BDNF using the BDNF Human ELISA kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews
A standard curve using the standards provided, homogenate of mouse cortex, and mouse serum were read in duplicate. Two blanks, where no sample was added, were also read.

1) 60 mg of cortex was lysed in 800uL of a buffer containing 100mM Tris, 150mM NaCl, 1mM EGTA, 1mM EDTA, 1% Triton, and protease inhibitors. The lysis was done at 4C rotating on a wheel for 2 hours.

2) lysate centrifuged at 16000xg for 20 minutes collect supernatant

3) cortex supertnatant was diluted 1:4 and serum was diluted 1:5 in Diluent A for the ELISA

4) The provided protocol was followed with the standards/samples being incubated with the plate at 4C overnight (step 2 of the Assay Method).

We were pleased with the results, as we have had trouble finding a reliable ELISA for mouse BDNF.
Username

Abcam user community

Verified customer

Submitted Jul 02 2013

Great products and option for mouse BDNF detection

Excellent Excellent 5/5 (Ease of Use)
Abreviews
This kit initially intended to detect human BDNF, successfully detect also mouse BDNF in brain samples. The kit is easy to use. Good product!
Username

Dr. Jianmin Wu

Verified customer

Submitted May 26 2016

Thank you for contacting us.



It is not necessary to filter the media, you can use it as is.





I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or i...

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During our QC testing, we tested normal serum and plasma samples with a 20 fold dilution to be ˜10ng/ml. As for the storage of the detection antibody, our guarantee for the kit is 6 months if stored at 2° to 8°C. Although not under the guarante...

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The capture and detection antibodies used in this kit both recognize a region within Arg128­-Arg247. Because the propeptide consists of amino acids 19 – 128, this kit would measure both mature and immature BDNF present in your sample.
Read More

The capture and detection antibodies used in this kit both recognize a region within Arg128­-Arg247. Because the propeptide consists of amino acids 19 – 128, this kit is only known to measure the mature form of BDNF. The molecular weight of t...

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Thank you for contacting us.

The molecular weight of the standard recombinant BDNF used in this kit (ELH-BDNF-001) is 26,984 Daltons. The sequence of the first five N-terminal amino acids of this BDNF was found to be Met-His-Ser-Asp-Pro. Acces...

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Thank you for your enquiry and interest in our products.

Guidelines to prepare urine samples

1. Collect urine without adding stabilizers.

2. Centrifuge the samples hard (eg. 10,000 x g for 1 min or 5,000 x g for 2 min). Read More

Thank you for your email which has been forwarded to me inthe technical support department.

I am happy to confirm that these kits should not be affected in their performanceif the packagewas not directly placed in therefrigeratorand thatthey ...

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Vielen Dank für Ihre Email und Ihren Anruf heute morgen.

Ich kann Ihnen bestätigen, dass beide Kits, ab99978 und ab100525, ohne Problem einen Tag bei Raumtemperatur aushalten können. Die Puffer und Antikörper können d...

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1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"