Save time with a 90-minute ELISA protocol. Try Human Beta-2-Microglobulin ELISA Kit (ab181423) from our SimpleStep ELISA® range.
Abcam’s beta 2 Microglobulin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of beta 2 Microglobulin levels in plasma, serum, milk, saliva, urine, CSF and cell culture supernatants.
A beta 2 Microglobulin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a beta 2 Microglobulin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of beta 2 Microglobulin captured in plate.
|Components||1 x 96 tests|
|100X Streptavidin-Peroxidase Conjugate||1 x 80µl|
|10X Diluent N Concentrate||1 x 30ml|
|20X Wash Buffer Concentrate||2 x 30ml|
|50X Biotinylated beta 2 Microglobulin Antibody||1 x 120µl|
|beta 2 Microglobulin Microplate (12 x 8 well strips)||1 unit|
|beta 2 Microglobulin Standard||1 x 60ng|
|Chromogen Substrate||1 x 8ml|
|Sealing Tapes||3 units|
|Stop Solution||1 x 12ml|
Our Abpromise guarantee covers the use of ab108885 in the following tested applications.
|Sandwich ELISA||Use at an assay dependent dilution.|
Standard curve: mean of duplicates (+/- SD) with background reads subtracted
Beta 2 Microglobulin measured in biological fluids and cell culture supernatants showing quantity (mU) per mL of tested sample. Human serum and plasma samples diluted 1000-8000 fold. Human milk diluted 4000-32000 fold. Human urine diluted 100-200 fold. Human saliva diluted 400-1600 fold. Mouse and rat samples diluted 10 fold. HepG2 supernatant diluted 1000 fold.
ab108885 has not yet been referenced specifically in any publications.