Human beta 2 Microglobulin ELISA Kit (B2M) (ab99977)

Overview

  • Product nameHuman beta 2 Microglobulin ELISA Kit (B2M)
    See all beta 2 Microglobulin kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 6 pg/ml
  • Range
    15.6 pg/ml - 1000 pg/ml
  • Recovery

    < 100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 104.6 93% - 118%
    Serum 94.28 80% - 103%
    Plasma 95.78 81% - 105%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s beta 2 Microglobulin (B2M) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human beta 2 Microglobulin in serum, plasma and cell culture supernatants.


    This assay employs an antibody specific for Human beta 2 Microglobulin coated on a 96-well plate. Standards and samples are pipetted into the wells and beta 2 Microglobulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human beta 2 Microglobulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of beta 2 Microglobulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    For higher sensitivity in only 90 minutes, we recommend Human Beta-2-Microglobulin ELISA Kit (ab181423) from our SimpleStep ELISA® range

  • Notes

    Optimisation may be required with urine samples.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • Storage instructionsStore at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    300X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 2 x 30ml
    beta 2 Microglobulin Microplate (12 x 8 wells) 1 unit
    Biotinylated anti-Human beta 2 Microglobulin 2 vials
    Recombinant Human beta 2 Microglobulin Standard 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas
  • RelevanceBeta 2 Microglobulin non-covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.
  • Cellular localizationSecreted
  • Alternative names
    • B2M
    • Beta 2 microglobulin precursor
    • Beta chain of mhc class 1 proteins
    • Hdcma22p
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab99977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human beta 2 Microglobulin ELISA Kit (B2M) images

  • huB2M measured in biofluids showing quantity (pg) per mL of tested sample

  • Representative Standard Curve using ab99977.

  • Representative Standard Curve using ab99977.

Protocols

References for Human beta 2 Microglobulin ELISA Kit (B2M) (ab99977)

ab99977 has not yet been referenced specifically in any publications.

Product Wall

This kit has not been tested for cross-reactivity with mouse samples. However, since human and mouse Beta-2M differ by 30% in their primary sequence it is unlikely this kit will detect mouse Beta 2M.

Thank you for contacting us.

As you mention, the kit can be stored up to 6 months at temperatures between 4-8°C.

The standard is stable at these temperatures as long as it has not been reconstituted.

Once reconstituted...

Read More

Thank you for your enquiry and your interest in our products.

Currently, we do have 2 kits for detecting human beta 2 Microglobulin i.e. ab99977 and ab108885.

I would advise you to read through the datasheets as well as the Protocol...

Read More

Thank you for contacting Abcam. I have been in contact with the labs about our kit and also have some additional details regarding our other Beta-2-Microglobulin products reactivity with porcine.

For our kits:

Regardingab99977:Unf...

Read More

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

In or...

Read More

Thank you for your inquiry.

I can confirm that serum/plasma samples usually require a 5,000˜100,000 fold dilution. I am sorry, I am unable to give a morenarrow range.

I agree that aserial dilutionis the best approach.

For ...

Read More

Thank you for your inquiry and you positive feedback.

Please see my answers to your questions below:

1. I can confirm that the kits ab108902 (Transferrin Human ELISA Kit) and ab108788 (Albumin Human ELISA kit) were especially opti...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"