Human Beta Nerve Growth Factor ELISA Kit (NGFB) (ab99986)
Key features and details
- Sensitivity: 14 pg/ml
- Range: 6.86 pg/ml - 5000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Beta Nerve Growth Factor ELISA Kit (NGFB)
See all Beta nerve growth factor kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 14 pg/ml -
Range
6.86 pg/ml - 5000 pg/ml -
Recovery
> 100 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 106.7 90% - 122% Serum 113.1 98% - 116% Plasma 102.8 85% - 117% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Beta Nerve Growth Factor (NGFB) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Beta Nerve Growth Factor in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human Beta Nerve Growth Factor coated on a 96-well plate. Standards and samples are pipetted into the wells and Beta Nerve Growth Factor present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Beta Nerve Growth Factor antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Beta Nerve Growth Factor bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get results in 90 minutes with Human beta Nerve Growth Factor ELISA Kit (ab193760) from our SimpleStep ELISA® range.
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Notes
NGFB concentration is very low in normal human serum samples and may be undetectable even with undiluted samples. Optimization may be required with urine samples.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml 800X HRP-Streptavidin Concentrate 1 x 200µl Beta Nerve Growth Factor Microplate (12 x 8 wells) 1 unit Beta Nerve Growth Factor Standard Human (recombinant) (lyophilized) 2 vials Biotinylated anti-Human Beta Nerve Growth Factor 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Relevance
Nerve growth factor (NGF) is one of a family of neurotrophins that induce the survival and proliferation of neurons. In cell culture, NGF induces the formation of neurite projections and, in vivo, may stimulate the innervation of tissues. NGF plays a role in the repair, regeneration, and protection of neurons, and as such could serve as a therapeutic agent in neurodegenerative conditions such as Alzheimer's disease. NGF enhances survival, growth, neurotransmitter biosynthesis of sympathetic and sensory neurons; neurotrophic factor; cutaneous innervation; growth, differentiation and survival of B lymphocytes. It also has a possible role in allergy and tissue repair. NGF is found in the hypothalamus, pituitary, thyroid gland, testes, epididymis, vascular smooth muscle cells, fibroblasts, mast cells and eosinophils. NGF is upregulated by glutamate, vitamin D3, IL6, FGF basic, astrocyte specific IL1, TNF alpha, PDGF and TGF beta. It is downregulated by GABAergic neuronal activity, glucocorticoids and Schwann cell-specific TGF beta. -
Cellular localization
Secreted. -
Alternative names
- Beta nerve growth factor
- Beta NGF
- HSAN5
see all -
Database links
- Entrez Gene: 4803 Human
- Omim: 162030 Human
- SwissProt: P01138 Human
Associated products
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SimpleStep ELISA kits
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab99986 has been referenced in 12 publications.
- Seidel MF et al. Localization of Nerve Growth Factor Expression to Structurally Damaged Cartilaginous Tissues in Human Lumbar Facet Joint Osteoarthritis. Front Immunol 13:783076 (2022). PubMed: 35300334
- Yoon EJ et al. Stamina-Enhancing Effects of Human Adipose-Derived Stem Cells. Cell Transplant 30:9636897211035409 (2021). PubMed: 34318707
- Kumar A et al. Localization and hypersecretion of nerve growth factor in breast phyllodes tumors: Evidence from a preliminary study. Cancer Rep (Hoboken) 4:e1300 (2021). PubMed: 33026185
- Bioque M et al. Acute effects of a session of electroconvulsive therapy on brain-derived neurotrophic factor plasma levels. Rev Psiquiatr Salud Ment N/A:N/A (2020). PubMed: 32674992
- Cen LP et al. Human Periodontal Ligament-Derived Stem Cells Promote Retinal Ganglion Cell Survival and Axon Regeneration After Optic Nerve Injury. Stem Cells 36:844-855 (2018). PubMed: 29476565