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|Sample type||Average %||Range|
|Cell culture supernatant||74.34||65% - 85%|
|Plasma||78.86||67% - 88%|
Abcam’s Betacellulin (BTC) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Betacellulin in plasma, and cell culture supernatants. (Human Betacellulin concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for Human Betacellulin coated on a 96-well plate. Standards and samples are pipetted into the wells and Betacellulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Betacellulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Betacellulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect the endogenous Human Betacellulin in normal serum with ab99987, only in serum spiked with Human Betacellulin.
Get higher sensitivity in only 90 minutes with Human Betacellulin ELISA Kit (ab189575) from our SimpleStep ELISA® range.
Optimization may be required with urine samples.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|240X HRP-Streptavidin Concentrate||1 x 200µl|
|5X Assay Diluent B||1 x 15ml|
|5X Assay Diluent D||1 x 15ml|
|Betacellulin Microplate (12 x 8 well strips)||1 unit|
|Betacellulin Standard Human (recombinant)||2 vials|
|Biotinylated anti-Human Betacellulin||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab99987 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab99987 has not yet been referenced specifically in any publications.