Human bladder tissue lysate (transitional cell carcinoma) - insoluble fraction (female, 45 years) (ab44657)

Overview

  • Product nameHuman bladder tissue lysate (transitional cell carcinoma) - insoluble fraction (female, 45 years)
    See all Bladder lysates
  • Description
    Human bladder insoluble fraction
  • General notes

    Location: Right wall of bladder at 6-9 o'clock Diagnosis: Transitional cell carcinoma, well differentiated Stage: III T3NXM0 Grade: I. Gross findings: Tumor irregular in shape, size 76 x 53mm. Cut section firm, non-identical and pink/red. Invasive and hemorrhagic. 

    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab44657: Extraction 1: soluble protein fraction ab44655 Human Bladder Tumor Tissue lysate ab44654 Human Bladder Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab44657 Human Bladder Tumor Tissue lysate ab44656 Human Bladder Normal Tissue lysate (matched)

Properties

  • FormLiquid
  • Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.40
    Constituents: PBS, 15.22% Thiourea, 30.03% Urea, 0.1% SDS, 0.77% DTT
  • Concentration information loading...
  • Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
  • Research areas

    References for Human bladder tissue lysate (transitional cell carcinoma) - insoluble fraction (female, 45 years) (ab44657)

    ab44657 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"