Human blood vessel: artery tissue lysate - total protein (ab28992)

Overview

  • Product nameHuman blood vessel: artery tissue lysate - total protein
    See all Blood Vessel: artery lysates
  • General notes


    Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot. Ethics: Human tissue samples are collected with the informed consent of the donors & relatives and are excised by licensed medical doctors. Tumor tissue samples are diagnosed and identified by at least two different evaluators. If you have more questions then please feel free to contact us. Donor information can be supplied on request.

  • Tested applicationsSuitable for: WBmore details

Properties

  • FormLiquid
  • Storage instructionsStore at -80°C. Allow to warm to room temp and agitate gently before aliquotting.
  • Storage bufferPreservative: None
    Constituents: 2% SDS, 10% Glycerol, 0.1% Bromophenol blue, Sodium Deoxycholate, Tergitol-NP40, EDTA, Magnesium chloride, HEPES, Potassium chloride, Protease inhibitor, 0.15M Beta mercaptoethanol, pH 7.9
  • Concentration information loading...
  • Lysate notesProtein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot. Ethics: Human tissue samples are collected with the informed consent of the donors & relatives and are excised by licensed medical doctors. Tumor tissue samples are diagnosed and identified by at least two different evaluators. If you have more questions then please feel free to contact us. Donor information can be supplied on request.
  • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab28992 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use.

    References for Human blood vessel: artery tissue lysate - total protein (ab28992)

    This product has been referenced in:
    • Pyla R  et al. Expression of conventional and novel glucose transporters, GLUT1, -9, -10, and -12, in vascular smooth muscle cells. Am J Physiol Cell Physiol 304:C574-89 (2013). Read more (PubMed: 23302780) »

    See 1 Publication for this product

    Product Wall

    Thank you for your enquiry and your valuable feedback. We will change the shipping conditions for these products as soon as possible. This is to confirm that we will ship again one vial of each of these lysates as free of charge replacements - on d...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"