Overview

  • Product name
    Human BNP ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    14 pg/ml
  • Range
    14 pg/ml - 1000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 78.12 68% - 83%
    Plasma 80.96 67% - 107%
    Cell culture media 94.31 83% - 105%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s BNP Human ELISA Kit (ab193694) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human BNP in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human BNP coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures BNP present in the samples. The wells are washed and biotinylated anti-Human BNP antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of BNP bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent C 1 x 30ml
    Biotinylated Human BNP Detection Antibody (lyophilized) 2 vials
    Human BNP Standards 2 vials
    Pre-coated Human BNP Microplate (12 strips x 8 wells) 1 unit
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • Function
    Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.
  • Tissue specificity
    Brain and also in atria, but at much lower levels than ANP.
  • Sequence similarities
    Belongs to the natriuretic peptide family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • ANFB_HUMAN
    • BNP(1-32)
    • BNP(5-29)
    • BNP-32
    • Brain type natriuretic peptide
    • Gamma-brain natriuretic peptide
    • Natriuretic peptide B
    • Natriuretic peptide precursor B
    • Natriuretic peptides B
    • Natriuretic protein
    • nppb
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab193694 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Standard curve using ab193694 BNP Human ELISA Kit.

    Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Protocols

References

ab193694 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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