Human Anti-Bordetella pertussis IgG ELISA Kit (ab108709)

Overview

  • Product nameHuman Anti-Bordetella pertussis IgG ELISA Kit
    See all Bordetella pertussis IgG kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Pos. Serum 20 3.9%
    Pos. Serum 24 5.2%
    Inter-assay
    Sample n Mean SD CV%
    Pos. Serum 12 4.9%
    Pos. Serum 12 6.1%
  • Tests
    1 x 96 test
  • Sample type
    Serum, Plasma
  • Assay typeIndirect
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s anti-Bordetella pertussis IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Bordetella pertussis in Human serum and plasma.

    A 96-well plate has been precoated with Bordetella pertussis antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Bordetella pertussis-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Bordetella pertussis IgG sample captured in plate.

  • Tested applicationsSuitable for: Indirect ELISAmore details
  • PlatformMicroplate

Properties

    Applications

    Our Abpromise guarantee covers the use of ab108709 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Indirect ELISA Use at an assay dependent concentration.

    Protocols

    References for Human Anti-Bordetella pertussis IgG ELISA Kit (ab108709)

    ab108709 has not yet been referenced specifically in any publications.

    Product Wall

    The pertussis toxin is the entire complex. The amount used for coating is FHA to PT diluted 1:2.

    The plates are coated with two proteins: Pertussis toxin and fillamentous hemagglutinin (FHA).

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"