Human Anti-Bordetella pertussis IgM ELISA Kit (ab108710)

Overview

  • Product nameHuman Anti-Bordetella pertussis IgM ELISA Kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Pos. Serum 20 8.5%
    Pos. Serum 24 14%
    Inter-assay
    Sample n Mean SD CV%
    Pos. Serum 12 9.4%
    Pos. Serum 12 8.2%
  • Tests
    1 x 96 test
  • Sample type
    Serum, Plasma
  • Assay typeIndirect
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s anti-Bordetella pertussis IgM Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Bordetella pertussis in Human serum and plasma.

     

    A 96-well plate has been precoated with Bordetella pertussis antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Bordetella pertussis-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Bordetella pertussis IgM sample captured in plate.

  • Tested applicationsSuitable for: Indirect ELISAmore details
  • PlatformMicroplate

Properties

    Applications

    Our Abpromise guarantee covers the use of ab108710 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Indirect ELISA Use at an assay dependent concentration.

    Protocols

    References for Human Anti-Bordetella pertussis IgM ELISA Kit (ab108710)

    ab108710 has not yet been referenced specifically in any publications.

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