Abcam’s anti-Borrelia burgdorferi IgM Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Borrelia burgdorferi in Human serum, plasma and CSF (cerebrospinal fluid).
A 96-well plate has been precoated with recombinant Borrelia burgdorferi antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Borrelia burgdorferi-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Borrelia burgdorferi IgM sample captured in plate.
|Components||Identifier||1 x 96 tests|
|20X Washing Solution||White cap||1 x 50ml|
|Borrelia burgdorferi (IgM) Coated Microplate (12 x 8 wells)||12 strips of 8 wells||1 unit|
|Borrelia burgdorferi anti-IgM HRP Conjugate||Red, Black cap||1 x 20ml|
|Borrelia burgdorferi IgM Cut-off Control||Yellow, Green cap||1 x 3ml|
|Borrelia burgdorferi IgM Negative Control||Yellow, Blue cap||1 x 2ml|
|Borrelia burgdorferi IgM Positive Control||Yellow, Red cap||1 x 2ml|
|Cover foil||1 unit|
|IgM Sample Diluent||Green, White cap||1 x 100ml|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|TMB Substrate Solution||Yellow cap||1 x 15ml|
Our Abpromise guarantee covers the use of ab108711 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent dilution.|