Human brain hippocampus tissue lysate - total protein (ab30180)

Overview

  • Product nameHuman brain hippocampus tissue lysate - total protein
    See all Brain: hippocampus lysates
  • Description
    Human brain total protein lysate
  • General notes


    Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot. Ethics: Human tissue samples are collected with the informed consent of the donors & relatives and are excised by licensed medical doctors. Tumor tissue samples are diagnosed and identified by at least two different evaluators. If you have more questions then please feel free to contact us. Donor information can be supplied on request.

  • Tested applicationsSuitable for: WBmore details

Properties

  • FormLiquid
  • Storage instructionsStore at -80°C. Allow to warm to room temp and agitate gently before aliquotting.
  • Storage bufferPreservative: None
    Constituents: 2% SDS, 10% Glycerol, 0.1% Bromophenol blue, Protease inhibitor, Sodium chloride, EDTA, Magnesium chloride, HEPES, 0.15M Beta mercaptoethanol
  • Concentration information loading...
  • Lysate notesProtein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot. Ethics: Human tissue samples are collected with the informed consent of the donors & relatives and are excised by licensed medical doctors. Tumor tissue samples are diagnosed and identified by at least two different evaluators. If you have more questions then please feel free to contact us. Donor information can be supplied on request.
  • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab30180 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use.

    References for Human brain hippocampus tissue lysate - total protein (ab30180)

    ab30180 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you very much for contacting me with this question.

    At this time we only have the the human hippocampal lysates that are prepared in sample buffer. We may be able to produce custom lysates that are only prepared in lysis buffer, however...

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    Thank you again for your email and for your patience while I have been in touch with the lab regarding your enquiry.

    Here is the information from the lab regarding the lots of ab30180 and ab30181 which you have recently received:

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    Thank you very much for your email.

    I will need to askthe lab for this information and I will be back in touch as soon as I have heard back. Please let me know if you have any further questions in the meantime.

    Have a nice day.

    Thank you for your patience. Please see below for donor information: 1) ab29661, LOT 536092: 75 Years / Male / Caucasian 2) ab29659, LOT 506051: 26 Years / Male / Asian 3) ab30027, LOT 682434: 87 Years / Male / Caucasian 4) ab30026, LOT 847628...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"