Human breast tissue lysate (ductal carcinoma) - insoluble fraction (female, 43 years) (ab43456)


  • Product nameHuman breast tissue lysate (ductal carcinoma) - insoluble fraction (female, 43 years)
    See all Breast lysates
  • Description
    Hu breast insoluble fraction lysate
  • General notesTissue: Breast Location: Right breast. Diagnosis: Invasive ductal carcinoma. Stage: IIA T2N0M0 Grade: III Sex: Female Age: 43 years Gross findings: Tumor size 3 x 4 x 4 cm. Cut section hard and white/gray. Ipsilateral axillary lymph nodes examined: 10 Microscopic findings: Cancer in surgical margin of section: Negative Multifocal carcinoma: Negative Pectoral muscular/facia invasion: Negative Chest wall invasion: Negative Dermal Lymphatic channel invasion: Negative Skin invasion with ulceration: Negative Dermal lymphatic channel invasion: Negative Lymphatic and/or blood vessel invasion: Negative Tumor necrosis: Positive Number of nodes positive: 0 Tubule formation: 3 Nuclear pleomorphism: 3 Mitotic activity: 1 Lymphocytic response: Positive Insoluble protein fraction

    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab43456: Extraction 1: soluble protein fraction ab43454 Human Breast Tumor Tissue lysate ab43453 Human Breast Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab43456 Human Breast Tumor Tissue lysate ab43455 Human Breast Normal Tissue lysate (matched)


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.40
    Constituents: PBS, 15.22% Thiourea, 30.03% Urea, 0.1% SDS, 0.77% DTT
  • Concentration information loading...
  • Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
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